« Previous AbstractAir Quality Impact of Diffuse and Inefficient Combustion Emissions in Africa (DICE-Africa)    Next AbstractAttraction of Amblyomma variegatum (ticks) to the attraction-aggregation-attachment-pheromone with or without carbon dioxide »

Indian J Microbiol

Title:		"Calmodulin, Calcium/Calmodulin-Dependent Kinases-1 and 2 Regulate Expression of the Heat Shock Proteins for Heat Shock Tolerance and Pheromone Signaling Genes for Sexual Development in Neurospora crassa"
Author(s):		Marak CNK; Tamuli R;
Address:		"Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781 039 India. ROR: https://ror.org/0022nd079. GRID: grid.417972.e. ISNI: 0000 0001 1887 8311"
Journal Title:		Indian J Microbiol
Year:		2023
Volume:		20230822
Issue:		3
Page Number:		317 - 323
DOI:		10.1007/s12088-023-01091-8
ISSN/ISBN:		0046-8991 (Print) 0973-7715 (Electronic) 0046-8991 (Linking)
Abstract:		"Calmodulin (CaM) is a primary Ca(2+) sensor that binds and activates numerous target proteins and regulates several cellular processes in eukaryotes. CaM is essential in Neurospora crassa; therefore, we generated a CaM mutant using repeat-induced point (RIP) mutation and investigated the cmd(RIP) mutant phenotypes. We also studied knockout mutants of four Ca(2+)/CaM kinases (camk-1, 2, 3, and 4) for their role during stress conditions and sexual development. The cmd(RIP), ?Oscamk-1, and ?Oscamk-2 mutants showed reduced survival and growth rates under heat stress, oxidative stress, pH, and ER stress conditions. In addition, under the heat stress conditions, expression of the heat shock protein genes hsp70 and hsp80 was reduced in the cmd(RIP), ?Oscamk-1, and ?Oscamk-2 mutants. The cmd(RIP) mutant was also defective in cell fusion, its vegetative hyphae could not support the fertilized wild type perithecia graft, and female sterile. Furthermore, the expression of pheromone signaling genes pre-1, pre-2, ccg-4, mfa-1, and fmf-1 was reduced in the cmd(RIP), ?Oscamk-1, and ?Oscamk-2 mutants. Therefore, CaM, Ca(2+)/CaMK-1 and 2 are involved in the tolerance to heat stress conditions and sexual development by regulating the heat shock and pheromone response pathways, respectively, in N. crassa. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12088-023-01091-8"
Keywords:		Ca2+/CaM kinases Cell fusion Heat shock protein 80 Perithecia grafting Pheromone signaling Sexual development Thermotolerance;
Notes:		"PubMed-not-MEDLINEMarak, Christy Noche K Tamuli, Ranjan eng India 2023/10/02 Indian J Microbiol. 2023 Sep; 63(3):317-323. doi: 10.1007/s12088-023-01091-8. Epub 2023 Aug 22"