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Plant J

Title:		Insect herbivory elicits genome-wide alternative splicing responses in Nicotiana attenuata
Author(s):		Ling Z; Zhou W; Baldwin IT; Xu S;
Address:		"Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, D-07745, Jena, Germany"
Journal Title:		Plant J
Year:		2015
Volume:		84
Issue:		1
Page Number:		228 - 243
DOI:		10.1111/tpj.12997
ISSN/ISBN:		1365-313X (Electronic) 0960-7412 (Linking)
Abstract:		"Changes in gene expression and alternative splicing (AS) are involved in many responses to abiotic and biotic stresses in eukaryotic organisms. In response to attack and oviposition by insect herbivores, plants elicit rapid changes in gene expression which are essential for the activation of plant defenses; however, the herbivory-induced changes in AS remain unstudied. Using mRNA sequencing, we performed a genome-wide analysis on tobacco hornworm (Manduca sexta) feeding-induced AS in both leaves and roots of Nicotiana attenuata. Feeding by M. sexta for 5 h reduced total AS events by 7.3% in leaves but increased them in roots by 8.0% and significantly changed AS patterns in leaves and roots of existing AS genes. Feeding by M. sexta also resulted in increased (in roots) and decreased (in leaves) transcript levels of the serine/arginine-rich (SR) proteins that are involved in the AS machinery of plants and induced changes in SR gene expression that were jasmonic acid (JA)-independent in leaves but JA-dependent in roots. Changes in AS and gene expression elicited by M. sexta feeding were regulated independently in both tissues. This study provides genome-wide evidence that insect herbivory induces changes not only in the levels of gene expression but also in their splicing, which might contribute to defense against and/or tolerance of herbivory"
Keywords:		"Alternative Splicing/genetics Animals Gene Expression Regulation, Plant/genetics Herbivory/*physiology Plant Proteins/genetics Tobacco/*genetics/*parasitology Manduca sexta Nicotiana attenuata alternative splicing herbivore-induced responses jasmonic acid;"
Notes:		"MedlineLing, Zhihao Zhou, Wenwu Baldwin, Ian T Xu, Shuqing eng 293926/ERC_/European Research Council/International Research Support, Non-U.S. Gov't England 2015/08/27 Plant J. 2015 Oct; 84(1):228-43. doi: 10.1111/tpj.12997"