| Title: | "Enterococcus faecalis pheromone binding protein, PrgZ, recruits a chromosomal oligopeptide permease system to import sex pheromone cCF10 for induction of conjugation" |
| Author(s): | Leonard BA; Podbielski A; Hedberg PJ; Dunny GM; |
| Address: | "Institute for Advanced Studies in Biological Process Technology, University of Minnesota Medical School, Minneapolis 55455, USA" |
| ISSN/ISBN: | 0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking) |
| Abstract: | "Conjugative transfer of the plasmid pCF10 by Enterococcus faecalis donor cells occurs in response to a peptide sex pheromone, cCF10, secreted by recipients. The plasmid-encoded cCF10 binding protein, PrgZ, is similar in sequence to binding proteins (OppAs) encoded by oligopeptide permease (opp) operons. Mutation of prgZ decreased the sensitivity of donor cells to pheromone, whereas inactivation of the chromosomal E. faecalis opp operon abolished response at physiological concentrations of pheromone. Affinity chromatography experiments demonstrated the interaction of the pheromone with several putative intracellular regulatory molecules, including an RNA molecule required for positive regulation of conjugation functions. These data suggest that processing of the pheromone signal involves recruitment of a chromosomal Opp system by PrgZ and that signaling occurs by direct interaction of internalized pheromone with intracellular effectors" |
| Keywords: | "Amino Acid Sequence Bacterial Proteins/genetics/*physiology Base Sequence Carrier Proteins/*metabolism *Conjugation, Genetic DNA Primers/chemistry Enterococcus faecalis/*physiology Macromolecular Substances Membrane Transport Proteins/*metabolism Molecula;" |
| Notes: | "MedlineLeonard, B A Podbielski, A Hedberg, P J Dunny, G M eng 1F32 AI08742/AI/NIAID NIH HHS/ 1RO1 GM49530/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 1996/01/09 Proc Natl Acad Sci U S A. 1996 Jan 9; 93(1):260-4. doi: 10.1073/pnas.93.1.260" |
