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Methods Mol Biol

Title:		Expression analysis of jasmonate-responsive lectins in plants
Author(s):		Lannoo N; Van Damme EJ;
Address:		"Laboratory of Glycobiology and Biochemistry, Department of Molecular Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1011
Issue:
Page Number:		251 - 263
DOI:		10.1007/978-1-62703-414-2_20
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"The Nicotiana tabacum lectin, also designated Nictaba, is a nucleocytoplasmic carbohydrate-binding protein produced in tobacco leaves after application of specific jasmonates and upon insect herbivory. Here, we describe different techniques by which lectin production can be induced through exogenous jasmonate application on tobacco plants. Furthermore, we elaborate on the assays to detect Nictaba expression at RNA and protein levels as well as on the agglutination assays to identify the lectin activity"
Keywords:		"Acetates/*pharmacology Blotting, Western Cyclopentanes/*pharmacology Enzyme-Linked Immunosorbent Assay Gene Expression *Gene Expression Profiling Gene Expression Regulation, Plant Oxylipins/*pharmacology Plant Growth Regulators/*pharmacology Plant Leaves/;"
Notes:		"MedlineLannoo, Nausicaa Van Damme, Els J M eng Research Support, Non-U.S. Gov't 2013/04/26 Methods Mol Biol. 2013; 1011:251-63. doi: 10.1007/978-1-62703-414-2_20"