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Molecules

Title:		Application of the Dehydration Homogeneous Liquid-Liquid Extraction (DHLLE) Sample Preparation Method for Fingerprinting of Honey Volatiles
Author(s):		Kus PM; Jerkovic I;
Address:		"Department of Pharmacognosy, Wroclaw Medical University, ul. Borowska 211a, 50-556 Wroclaw, Poland. Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, Rudera Boskovica 35, 21000 Split, Croatia"
Journal Title:		Molecules
Year:		2021
Volume:		20210414
Issue:		8
Page Number:		-
DOI:		10.3390/molecules26082277
ISSN/ISBN:		1420-3049 (Electronic) 1420-3049 (Linking)
Abstract:		"Recently, we proposed a new sample preparation method involving reduced solvent and sample usage, based on dehydration homogeneous liquid-liquid extraction (DHLLE) for the screening of volatiles and semi-volatiles from honey. In the present research, the method was applied to a wide range of honeys (21 different representative unifloral samples) to determine its suitability for detecting characteristic honey compounds from different chemical classes. GC-FID/MS disclosed 130 compounds from different structural and chemical groups. The DHLLE method allowed the extraction and identification of a wide range of previously reported specific and nonspecific marker compounds belonging to different chemical groups (including monoterpenes, norisoprenoids, benzene derivatives, or nitrogen compounds). For example, DHLLE allowed the detection of cornflower honey chemical markers: 3-oxo-retro-alpha-ionols, 3,4-dihydro-3-oxoedulan, phenyllactic acid; coffee honey markers: theobromine and caffeine; linden honey markers: 4-isopropenylcyclohexa-1,3-diene-1-carboxylic acid and 4-(2-hydroxy-2-propanyl)cyclohexa-1,3-diene-1-carboxylic acid, as well as furan derivatives from buckwheat honey. The obtained results were comparable with the previously reported data on markers of various honey varieties. Considering the application of much lower volumes of very common reagents, DHLLE may provide economical and ecological advantages as an alternative sample preparation method for routine purposes"
Keywords:		*Analytic Sample Preparation Methods Dehydration Gas Chromatography-Mass Spectrometry Honey/*analysis Liquid-Liquid Extraction/*methods Solid Phase Extraction Solvents/chemistry Ultrasonics Volatile Organic Compounds/*analysis green extraction honey chemi;
Notes:		"MedlineKus, Piotr M Jerkovic, Igor eng DEC-2014/15/N/NZ9/04058/Narodowe Centrum Nauki/ Switzerland 2021/05/01 Molecules. 2021 Apr 14; 26(8):2277. doi: 10.3390/molecules26082277"