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Methods Mol Biol

Title:		Murine nonvolatile pheromones: isolation of exocrine-gland secreting Peptide 1
Author(s):		Kimoto H; Touhara K;
Address:		"Biological Research Laboratories, R&D Division, Daiichi Sankyo Co., LTD., Tokyo, Japan"
Journal Title:		Methods Mol Biol
Year:		2013
Volume:		1068
Issue:
Page Number:		47 - 53
DOI:		10.1007/978-1-62703-619-1_4
ISSN/ISBN:		1940-6029 (Electronic) 1064-3745 (Linking)
Abstract:		"Our search for a substance recognized by the vomeronasal neurons revealed that the extra-orbital lacrimal gland (ELG) isolated from adult male mice produced the male-specific peptide pheromone exocrine gland-secreting peptide 1 (ESP1). The following protocol reveals how ESP1 may be extracted from the ELG, purified using anion-exchange and reverse-phase high-performance liquid chromatography (HPLC), and analyzed by mass spectrometry. This protocol has been specifically designed for the purification of ESP1, but may be modified to isolate a variety of peptides from the exocrine glands. Peptides purified in this manner may help further define the molecular mechanisms underlying pheromone communication in the vomeronasal system"
Keywords:		"Animals Chromatography, High Pressure Liquid/*methods Chromatography, Reverse-Phase/*methods Intercellular Signaling Peptides and Proteins Lacrimal Apparatus/metabolism Male Mass Spectrometry/*methods Mice Mice, Inbred BALB C Pheromones/*analysis/chemistr;"
Notes:		"MedlineKimoto, Hiroko Touhara, Kazushige eng 2013/09/10 Methods Mol Biol. 2013; 1068:47-53. doi: 10.1007/978-1-62703-619-1_4"