| Title: | "Metabolic Profiling of Hybrids Generated from Pummelo and Citrus latipes in Relation to Their Attraction to Diaphorina citri, the Vector of Huanglongbing" |
| Author(s): | Killiny N; Jones SE; Hijaz F; Kishk A; Santos-Ortega Y; Nehela Y; Omar AA; Yu Q; Gmitter FG; Grosser JW; Dutt M; |
| Address: | "Department of Plant Pathology, University of Florida, Citrus Research and Education Center, IFAS, Lake Alfred, FL 33850, USA. Department of Plant Protection, Faculty of Agriculture, Tanta University, Tanta 31511, Egypt. Department of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31511, Egypt. Department of Horticultural Sciences, University of Florida, Citrus Research and Education Center, IFAS, Lake Alfred, FL 33850, USA. Biochemistry Department, College of Agriculture, Zagazig University, Zagazig 44519, Egypt" |
| ISSN/ISBN: | 2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking) |
| Abstract: | "The citrus industry at present is severely affected by huanglongbing disease (HLB). HLB is caused by the supposed bacterial pathogen 'Candidatus Liberibacter asiaticus' and is transmitted by the insect vector, the Asian citrus psyllid, Diaphorina citri Kuwayama. Developing new citrus hybrids to improve HLB management is much needed. In this study, we investigated the metabolomic profiles of three new hybrids produced from the cross of C2-5-12 Pummelo (Citrus maxima (L.) Osbeck) x pollen from Citrus latipes. The hybrids were selected based on leaf morphology and seedling vigor. The selected hybrids exhibited compact and upright tree architecture as seen in C. latipes. Hybrids were verified by simple sequence repeat markers, and were subjected to metabolomic analysis using gas chromatography-mass spectrometry. The volatile organic compounds (VOCs) and polar metabolites profiling also showed that the new hybrids were different from their parents. Interestingly, the levels of stored VOCs in hybrid II were higher than those observed in its parents and other hybrids. The level of most VOCs released by hybrid II was also higher than that released from its parents. Additionally, the preference assay showed that hybrid II was more attractive to D. citri than its parents and other hybrids. The leaf morphology, compact and upright architecture of hybrid II, and its attraction to D. citri suggest that it could be used as a windbreak and trap tree for D. citri (double duty), once its tolerance to HLB disease is confirmed. Our results showed that metabolomic analysis could be successfully used to understand the biochemical mechanisms controlling the interaction of D. citri with its host plants" |
| Keywords: | Asian citrus psyllids Citrus latipes Pummelo citrus breeding volatile organic compounds; |
| Notes: | "PubMed-not-MEDLINEKilliny, Nabil Jones, Shelley E Hijaz, Faraj Kishk, Abdelaziz Santos-Ortega, Yulica Nehela, Yasser Omar, Ahmad A Yu, Qibin Gmitter, Fred G Jr Grosser, Jude W Dutt, Manjul eng 19-015/Citrus Research and Development Foundation/ Switzerland 2020/12/02 Metabolites. 2020 Nov 24; 10(12):477. doi: 10.3390/metabo10120477" |
