| Title: | Integrated metabolome and transcriptome analysis provides insights on the floral scent formation in Hydrangea arborescens |
| Author(s): | Ke Y; Zhou Y; Lv Y; Qi Y; Wei H; Lei Y; Huang F; Abbas F; |
| Address: | "College of Economics and Management, Kunming University, Kunming, China. Yunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, China. Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization, Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops-South China/Guangdong Litchi Engineering Research Center, College of Horticulture, South China Agricultural University, Guangzhou, China" |
| ISSN/ISBN: | 1399-3054 (Electronic) 0031-9317 (Linking) |
| Abstract: | "Hydrangea (Hydrangea arborescens var. 'Annabelle') flowers are composed of sweet aroma sepals rather than true petals and can change color. Floral volatiles plays important roles in plants, such as attracting pollinators, defending against herbivores, and signaling. However, the biosynthesis and regulatory mechanisms underlying fragrance formation in H. arborescens during flower development remain unknown. In this study, a combination of metabolite profiling and RNA sequencing (RNA-seq) was employed to identify genes associated with floral scent biosynthesis mechanisms in 'Annabelle' flowers at three developmental stages (F1, F2, and F3). The floral volatile data revealed that the 'Annabelle' volatile profile includes a total of 33 volatile organic compounds (VOCs), and VOCs were abundant during the F2 stage of flower development, followed by the F1 and F3 stages, respectively. Terpenoids and benzenoids/phenylpropanoids were abundant during the F2 and F1 stages, with the latter being the most abundant, whereas fatty acid derivatives and other compounds were found in large amounts during the F3 stage. According to ultra-performance liquid chromatography-tandem mass spectrometer analysis, benzene and substituted derivatives, carboxylic acids and derivatives, and fatty acyls play a significant role in the floral metabolite profile. The transcriptome data revealed a total of 17,461 differentially expressed genes (DEGs), with 7585, 12,795, and 9044 DEGs discovered between the F2 and F1, F3 and F1, and F2 and F3 stages, respectively. Several terpenoids and benzenoids/phenylpropanoids biosynthesis-related DEGs were identified, and GRAS/bHLH/MYB/AP2/WRKY were more abundant among transcription factors. Finally, DEGs interlinked with VOCs compounds were determined using Cytoscape and k-means analysis. Our results pave the way for the discovery of new genes, critical data for future genetic studies, and a platform for the metabolic engineering of genes involved in the production of Hydrangea's signature floral fragrance" |
| Keywords: | *Hydrangea/genetics/metabolism Odorants Gene Expression Profiling/methods Terpenes/metabolism Transcriptome Metabolome Flowers/metabolism; |
| Notes: | "MedlineKe, Yanguo Zhou, Yiwei Lv, Yiying Qi, Ying Wei, Huanyu Lei, Yu Huang, Feiyan Abbas, Farhat eng 202101BA070001-166/Yunnan Provincial Science and Technology Department Local Universities Joint Project/ XJ20220003/Talent Introduction Project of Kunming University/ 2022J0644/Science and Technology Project of Yunnan Provincial Education Department/ Denmark 2023/04/19 Physiol Plant. 2023 May-Jun; 175(3):e13914. doi: 10.1111/ppl.13914" |
