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Plant Physiol Biochem

Title:		Identification and characterization of (E)-beta-caryophyllene synthase and alpha/beta-pinene synthase potentially involved in constitutive and herbivore-induced terpene formation in cotton
Author(s):		Huang X; Xiao Y; Kollner TG; Zhang W; Wu J; Wu J; Guo Y; Zhang Y;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; College of Plant Protection, Northwest A & F University, Yangling, Shaanxi, China. Electronic address: huangxinzheng@nwsuaf.edu.cn"
Journal Title:		Plant Physiol Biochem
Year:		2013
Volume:		20131017
Issue:
Page Number:		302 - 308
DOI:		10.1016/j.plaphy.2013.10.017
ISSN/ISBN:		1873-2690 (Electronic) 0981-9428 (Linking)
Abstract:		"Cotton (Gossypium hirsutum L.) plants damaged by insects emit a blend of volatiles, including monoterpenes and sesquiterpenes, which can directly repel herbivores and/or indirectly protect the plant by attracting natural enemies of the herbivores. To understand the molecular basis of terpene biosynthesis and regulation in cotton, two terpene synthase genes, GhTPS1 and GhTPS2, were heterologously expressed and characterized. Recombinant GhTPS1 accepted farnesyl pyrophosphate as substrate and produced (E)-beta-caryophyllene and alpha-humulene. GhTPS2 was characterized as a monoterpene synthase which formed alpha-pinene and beta-pinene using geranyl pyrophosphate as substrate. Quantitative real-time PCR analysis revealed that GhTPS1 and GhTPS2 gene expression was elevated after methyl jasmonate (MeJA) treatment in cotton leaves. Moreover, feeding of the green plant bug Apolygus lucorum, a major cotton pest in northern China, resulted in increased GhTPS2 expression in young leaves, suggesting that GhTPS2 might be involved in plant defense in cotton"
Keywords:		"Acetates/metabolism/pharmacology Adaptation, Physiological Alkyl and Aryl Transferases/*genetics/metabolism Animals Bicyclic Monoterpenes Bridged Bicyclo Compounds/metabolism Carbon-Oxygen Lyases/genetics/metabolism China Cyclopentanes/metabolism/pharmaco;"
Notes:		"MedlineHuang, Xinzheng Xiao, Yutao Kollner, Tobias G Zhang, Wanna Wu, Junxiang Wu, Juan Guo, Yuyuan Zhang, Yongjun eng Research Support, Non-U.S. Gov't France 2013/11/05 Plant Physiol Biochem. 2013 Dec; 73:302-8. doi: 10.1016/j.plaphy.2013.10.017. Epub 2013 Oct 17"