| Title: | "High-throughput ESI-MS analysis of binding between the Bombyx mori pheromone-binding protein BmorPBP1, its pheromone components and some analogues" |
| Author(s): | Hooper AM; Dufour S; He X; Muck A; Zhou JJ; Almeida R; Field LM; Svatos A; Pickett JA; |
| Address: | "Rothamsted Research, Department of Biological Chemistry, West Common, Harpenden, Hertfordshire, UK AL5 2JQ. tony.hooper@bbsrc.ac.uk" |
| ISSN/ISBN: | 1364-548X (Electronic) 1359-7345 (Linking) |
| Abstract: | "Chip-assisted high-throughput ESI-MS analysis of the pheromone-binding protein of the silkworm moth Bombyx mori, BmorPBP1, incubated with its pheromone components bombykol, bombykal and analogues was developed. The protein bound to bombykol ((10E,12Z)-hexadecadien-1-ol) and all 3 of its geometric isomers to a lesser extent, and showed relaxed specificity toward different chain lengths possessing unsaturation. BmorPBP1 did not bind to bombykal ((10E,12Z)-hexadecadienal), demonstrating molecular recognition of the insect pheromone components" |
| Keywords: | "Alkadienes/chemistry Animals Bombyx/*chemistry Carrier Proteins/*chemistry Fatty Alcohols/chemistry Insect Proteins/*chemistry Intercellular Signaling Peptides and Proteins Pheromones/*chemistry Protein Binding *Spectrometry, Mass, Electrospray Ionization;" |
| Notes: | "MedlineHooper, Antony M Dufour, Samuel He, Xaoli Muck, Alexander Zhou, Jing-Jiang Almeida, Reinaldo Field, Linda M Svatos, Ales Pickett, John A eng Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2009/09/24 Chem Commun (Camb). 2009 Oct 14; (38):5725-7. doi: 10.1039/b914294k. Epub 2009 Sep 4" |
