| Title: | Analysis of Exhaled Breath Volatile Organic Compounds in Inflammatory Bowel Disease: A Pilot Study |
| Author(s): | Hicks LC; Huang J; Kumar S; Powles ST; Orchard TR; Hanna GB; Williams HR; |
| Address: | "Gastroenterology & Hepatology Section, Department of Medicine, Imperial College London, London UK. Department of Surgery and Cancer, Imperial College London, London UK" |
| ISSN/ISBN: | 1876-4479 (Electronic) 1873-9946 (Linking) |
| Abstract: | "BACKGROUND AND AIMS: Distinguishing between the inflammatory bowel diseases [IBD], Crohn's disease [CD] and ulcerative colitis [UC], is important for determining management and prognosis. Selected ion flow tube mass spectrometry [SIFT-MS] may be used to analyse volatile organic compounds [VOCs] in exhaled breath: these may be altered in disease states, and distinguishing breath VOC profiles can be identified. The aim of this pilot study was to identify, quantify, and analyse VOCs present in the breath of IBD patients and controls, potentially providing insights into disease pathogenesis and complementing current diagnostic algorithms. METHODS: SIFT-MS breath profiling of 56 individuals [20 UC, 18 CD, and 18 healthy controls] was undertaken. Multivariate analysis included principal components analysis and partial least squares discriminant analysis with orthogonal signal correction [OSC-PLS-DA]. Receiver operating characteristic [ROC] analysis was performed for each comparative analysis using statistically significant VOCs. RESULTS: OSC-PLS-DA modelling was able to distinguish both CD and UC from healthy controls and from one other with good sensitivity and specificity. ROC analysis using combinations of statistically significant VOCs [dimethyl sulphide, hydrogen sulphide, hydrogen cyanide, ammonia, butanal, and nonanal] gave integrated areas under the curve of 0.86 [CD vs healthy controls], 0.74 [UC vs healthy controls], and 0.83 [CD vs UC]. CONCLUSIONS: Exhaled breath VOC profiling was able to distinguish IBD patients from controls, as well as to separate UC from CD, using both multivariate and univariate statistical techniques" |
| Keywords: | "Adult Aged Aged, 80 and over Biomarkers/metabolism Breath Tests Case-Control Studies Colitis, Ulcerative/*diagnosis/metabolism Crohn Disease/*diagnosis/metabolism Diagnosis, Differential Discriminant Analysis Female Humans Male Mass Spectrometry/methods M;" |
| Notes: | "MedlineHicks, Lucy C Huang, Juzheng Kumar, Sacheen Powles, Sam T Orchard, Timothy R Hanna, George B Williams, Horace R T eng Research Support, Non-U.S. Gov't England 2015/06/14 J Crohns Colitis. 2015 Sep; 9(9):731-7. doi: 10.1093/ecco-jcc/jjv102. Epub 2015 Jun 12" |
