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J Chromatogr A

Title:		Determination of disease biomarkers in Eucalyptus by comprehensive two-dimensional gas chromatography and multivariate data analysis
Author(s):		Hantao LW; Aleme HG; Passador MM; Furtado EL; Ribeiro FA; Poppi RJ; Augusto F;
Address:		"Institute of Chemistry - University of Campinas (Unicamp), CP 6154, 13084-970 Campinas, Sao Paulo, Brazil"
Journal Title:		J Chromatogr A
Year:		2013
Volume:		20130112
Issue:
Page Number:		86 - 91
DOI:		10.1016/j.chroma.2013.01.013
ISSN/ISBN:		1873-3778 (Electronic) 0021-9673 (Linking)
Abstract:		"In this paper is reported the use of the chromatographic profiles of volatiles to determine disease markers in plants - in this case, leaves of Eucalyptus globulus contaminated by the necrotroph fungus Teratosphaeria nubilosa. The volatile fraction was isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography-fast quadrupole mass spectrometry (GCxGC-qMS). For the correlation between the metabolic profile described by the chromatograms and the presence of the infection, unfolded-partial least squares discriminant analysis (U-PLS-DA) with orthogonal signal correction (OSC) were employed. The proposed method was checked to be independent of factors such as the age of the harvested plants. The manipulation of the mathematical model obtained also resulted in graphic representations similar to real chromatograms, which allowed the tentative identification of more than 40 compounds potentially useful as disease biomarkers for this plant/pathogen pair. The proposed methodology can be considered as highly reliable, since the diagnosis is based on the whole chromatographic profile rather than in the detection of a single analyte"
Keywords:		"Ascomycota/physiology Biomarkers/*analysis/metabolism Chromatography, Gas/instrumentation/*methods Eucalyptus/*chemistry/metabolism/microbiology Multivariate Analysis Plant Diseases/*microbiology Plant Leaves/chemistry/metabolism/microbiology Volatile Org;"
Notes:		"MedlineHantao, Leandro Wang Aleme, Helga Gabriela Passador, Martha Maria Furtado, Edson Luiz Ribeiro, Fabiana Alves de Lima Poppi, Ronei Jesus Augusto, Fabio eng Evaluation Study Research Support, Non-U.S. Gov't Netherlands 2013/02/12 J Chromatogr A. 2013 Mar 1; 1279:86-91. doi: 10.1016/j.chroma.2013.01.013. Epub 2013 Jan 12"