| Title: | Giant vesicles functionally expressing membrane receptors for an insect pheromone |
| Author(s): | Hamada S; Tabuchi M; Toyota T; Sakurai T; Hosoi T; Nomoto T; Nakatani K; Fujinami M; Kanzaki R; |
| Address: | "Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, Chiba University, 1-33 Yayoi, Inage, Chiba, Chiba 263-8522, Japan. fujinami@faculty.chiba-u.jp" |
| ISSN/ISBN: | 1364-548X (Electronic) 1359-7345 (Linking) |
| Abstract: | "To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone" |
| Keywords: | "Animals Bombyx Fatty Alcohols/metabolism Green Fluorescent Proteins Insect Proteins/genetics/*metabolism Receptors, Odorant/genetics/*metabolism Receptors, Pheromone/genetics/*metabolism Sex Attractants/metabolism;" |
| Notes: | "MedlineHamada, Satoshi Tabuchi, Masashi Toyota, Taro Sakurai, Takeshi Hosoi, Tomohiro Nomoto, Tomonori Nakatani, Kei Fujinami, Masanori Kanzaki, Ryohei eng Research Support, Non-U.S. Gov't England 2014/02/11 Chem Commun (Camb). 2014 Mar 18; 50(22):2958-61. doi: 10.1039/c3cc48216b. Epub 2014 Feb 7" |
