| Title: | Glycerol-3-phosphate O-acyltransferase is required for PBAN-induced sex pheromone biosynthesis in Bombyx mori |
| Author(s): | Du M; Liu X; Liu X; Yin X; Han S; Song Q; An S; |
| Address: | "State key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou 450002 P.R. China. Translational Research Center, Zhengzhou University People's Hospital, Zhengzhou 450003 P.R. China. Division of Plant Sciences, University of Missouri, Columbia, Missouri, United States of America" |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Female moths employ their own pheromone blends as a communicational medium in mating behavior. The biosynthesis and release of sex pheromone in female moths are regulated by pheromone biosynthesis activating neuropeptide (PBAN) and the corresponding action of PBAN has been well elucidated in Bombyx mori. However, very little is known about the molecular mechanism regarding the biosynthesis of sex pheromone precursor. In this study, quantitative proteomics was utilized to comprehensively elucidate the expression dynamics of pheromone glands (PGs) during development. Proteomic analysis revealed a serial of differentially expressed sex pheromone biosynthesis-associated proteins at the different time points of B. mori development. Most interestingly B. mori glycerol-3-phosphate O-acyltransferase (BmGPAT) was found to be expressed during the key periods of sex pheromone biosynthesis. RNAi knockdown of BmGPAT confirmed the important function of this protein in the biosynthesis of sex pheromone precursor, triacylglcerol (TAG), and subsequently PBAN-induced production of sex pheromone, bombykol. Behavioral analysis showed that RNAi knockdown of GPAT significantly impaired the ability of females to attract males. Our findings indicate that GPAT acts to regulate the biosynthesis of sex pheromone precursor, TAG, thus influencing PBAN-induced sex pheromone production and subsequent mating behavior" |
| Keywords: | Amino Acid Sequence Animal Structures/metabolism Animals Bombyx/drug effects/*enzymology/genetics/growth & development Fatty Alcohols/metabolism Female Gene Expression Regulation/drug effects Gene Knockdown Techniques Gene Ontology Glycerol-3-Phosphate O-; |
| Notes: | "MedlineDu, Mengfang Liu, Xiaoguang Liu, Xiaoming Yin, Xinming Han, Shuangyin Song, Qisheng An, Shiheng eng Research Support, Non-U.S. Gov't England 2015/01/30 Sci Rep. 2015 Jan 29; 5:8110. doi: 10.1038/srep08110" |
