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ACS Synth Biol

Title:		Bacillus subtilis biosensor engineered to assess meat spoilage
Author(s):		Daszczuk A; Dessalegne Y; Drenth I; Hendriks E; Jo E; van Lente T; Oldebesten A; Parrish J; Poljakova W; Purwanto AA; van Raaphorst R; Boonstra M; van Heel A; Herber M; van der Meulen S; Siebring J; Sorg RA; Heinemann M; Kuipers OP; Veening JW;
Address:		"iGEM Teaching Program, Team 2012, double daggerMolecular Genetics Group, and section signMolecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen , 9747 AG Groningen, The Netherlands"
Journal Title:		ACS Synth Biol
Year:		2014
Volume:		3
Issue:		12
Page Number:		999 - 1002
DOI:		10.1021/sb5000252
ISSN/ISBN:		2161-5063 (Electronic) 2161-5063 (Linking)
Abstract:		"Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was PsboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned PsboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector. We show that the newly identified promoter could efficiently drive fluorescent protein production in B. subtilis in response to spoiled meat and thus can be used as a biosensor to detect meat spoilage"
Keywords:		"Bacillus subtilis/chemistry/*genetics/*metabolism Bacteriocins/genetics/metabolism Biosensing Techniques/*methods Fluorescent Dyes Gene Expression Profiling Genetic Engineering/*methods Meat/*analysis Peptides, Cyclic/genetics/metabolism Plasmids Syntheti;"
Notes:		"MedlineDaszczuk, Alicja Dessalegne, Yonathan Drenth, Ismael Hendriks, Elbrich Jo, Emeraldo van Lente, Tom Oldebesten, Arjan Parrish, Jonathon Poljakova, Wlada Purwanto, Annisa A van Raaphorst, Renske Boonstra, Mirjam van Heel, Auke Herber, Martijn van der Meulen, Sjoerd Siebring, Jeroen Sorg, Robin A Heinemann, Matthias Kuipers, Oscar P Veening, Jan-Willem eng Research Support, Non-U.S. Gov't 2014/12/20 ACS Synth Biol. 2014 Dec 19; 3(12):999-1002. doi: 10.1021/sb5000252"