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Plants (Basel)

Title:		Production of the Green Leaf Volatile (Z)-3-Hexenal by a Zea mays Hydroperoxide Lyase
Author(s):		Yactayo-Chang JP; Hunter CT; Alborn HT; Christensen SA; Block AK;
Address:		"Chemistry Research Unit, Center for Medical, Agricultural and Veterinary Entomology, USDA-Agricultural Research Service, Gainesville, FL 32608, USA"
Journal Title:		Plants (Basel)
Year:		2022
Volume:		20220825
Issue:		17
Page Number:		-
DOI:		10.3390/plants11172201
ISSN/ISBN:		2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking)
Abstract:		"Plant-produced volatile compounds play important roles in plant signaling and in the communication of plants with other organisms. Many plants emit green leaf volatiles (GLVs) in response to damage or attack, which serve to warn neighboring plants or attract predatory or parasitic insects to help defend against insect pests. GLVs include aldehydes, esters, and alcohols of 6-carbon compounds that are released rapidly following wounding. One GLV produced by maize (Zea mays) is the volatile (Z)-3-hexenal; this volatile is produced from the cleavage of (9Z,11E,15Z)-octadecatrienoic acid by hydroperoxide lyases (HPLs) of the cytochrome P450 CYP74B family. The specific HPL in maize involved in (Z)-3-hexenal production had not been determined. In this study, we used phylogenetics with known HPLs from other species to identify a candidate HPL from maize (ZmHPL). To test the ability of the putative HPL to produce (Z)-3-hexenal, we constitutively expressed the gene in Arabidopsis thaliana ecotype Columbia-0 that contains a natural loss-of-function mutant in AtHPL and examined the transgenic plants for restored (Z)-3-hexenal production. Volatile analysis of leaves from these transgenic plants showed that they did produce (Z)-3-hexenal, confirming that ZmHPL can produce (Z)-3-hexenal in vivo. Furthermore, we used gene expression analysis to show that expression of ZmHPL is induced in maize in response to both wounding and the insect pests Spodoptera frugiperda and Spodoptera exigua. Our study demonstrates that ZmHPL can produce GLVs and highlights its likely role in (Z)-3-hexenal production in response to mechanical damage and herbivory in maize"
Keywords:		Arabidopsis Glv Hpl armyworm defense insect maize wounding;
Notes:		"PubMed-not-MEDLINEYactayo-Chang, Jessica P Hunter, Charles T Alborn, Hans T Christensen, Shawn A Block, Anna K eng 6036-11210-001-00D/Agricultural Research Service/ 2018-51181-28419/National Institute of Food and Agriculture/ Switzerland 2022/09/10 Plants (Basel). 2022 Aug 25; 11(17):2201. doi: 10.3390/plants11172201"