| Title: | Study of the Vapor Phase Over Fusarium Fungi Cultured on Various Substrates |
| Author(s): | Savelieva EI; Gustyleva LK; Kessenikh ED; Khlebnikova NS; Leffingwell J; Gavrilova OP; Gagkaeva TY; |
| Address: | "Research Institute of Hygiene, Occupational Pathology and Human Ecology Federal State Unitary Enterprise, Federal Medical Biological Agency, Build. 93, Kapitolovo Station, Kuz'molovsky g/p, Vsevolozhsky District, Leningrad Region, RU-188663. Research Institute of Hygiene, Occupational Pathology and Human Ecology Federal State Unitary Enterprise, Federal Medical Biological Agency, Build. 93, Kapitolovo Station, Kuz'molovsky g/p, Vsevolozhsky District, Leningrad Region, RU-188663. esavelieva59@mail.ru. Leffingwell & Associates, 4699 Arbor Hill Rd., Canton, GA, 30115, USA. leffingwell@mindspring.com. All-Russian Institute of Plant Protection (VIZR) Podbelskogo shosse, 3 St.-Petersburg, Pushkin, RU-196608" |
| ISSN/ISBN: | 1612-1880 (Electronic) 1612-1872 (Linking) |
| Abstract: | "The compositions of volatile organic compounds (VOCs) emitted by Fusarium fungi (F. langsethiae, F. sibiricum, F. poae, and F. sporotrichioides) grown on two nutritive substrates: potato sucrose agar (PSA) and autoclaved wheat kernels (WK) were investigated. The culturing of fungi and study of their VOC emissions were performed in chromatographic vials at room temperature (23 - 24 degrees C) and the VOCs were sampled by a solid-phase microextraction on a 85 mum carboxen/polydimethylsiloxane fiber. GC/MS was performed using a 60-m HP-5 capillary column. Components of the VOC mixture were identified by electron impact mass spectra and chromatographic retention indices (RIs). The most abundant components of the VOC mixture emitted by Fusarium fungi are EtOH, AcOH, (i) BuOH, 3-methylbutan-1-ol, 2-methylbutan-1-ol, ethyl 3-methylbutanoate, terpenes with M 136, sesquiterpenes with M 204 (a total of about 25), and trichodiene. It was found that the strains grown on PSA emit a wider spectrum and larger amount of VOCs compared with those grown on wheat kernels. F. langsethiae strain is the most active VOC producer on both substrates. The use of SPME and GC/MS also offers the potential for differentiation of fungal species and strains" |
| Keywords: | Agar/metabolism Fusarium/*growth & development/*metabolism Gas Chromatography-Mass Spectrometry Gases/*analysis/chemistry/metabolism Seeds/metabolism Solanum tuberosum/chemistry Solid Phase Microextraction Sucrose/metabolism Triticum Volatile Organic Comp; |
| Notes: | "MedlineSavelieva, Elena I Gustyleva, Liudmila K Kessenikh, Elizaveta D Khlebnikova, Natalya S Leffingwell, John Gavrilova, Olga P Gagkaeva, Tatiana Yu eng Switzerland 2016/06/03 Chem Biodivers. 2016 Jul; 13(7):891-903. doi: 10.1002/cbdv.201500284. Epub 2016 Jul 4" |
