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J Allergy Clin Immunol

Title:		Tissue localization of bovine dander allergen Bos d 2
Author(s):		Rautiainen J; Rytkonen M; Syrjanen K; Pentikainen J; Zeiler T; Virtanen T; Mantyjarvi R;
Address:		"Department of Clinical Microbiology, University of Kuopio, Finland"
Journal Title:		J Allergy Clin Immunol
Year:		1998
Volume:		101
Issue:		3
Page Number:		349 - 353
DOI:		10.1016/S0091-6749(98)70247-7
ISSN/ISBN:		0091-6749 (Print) 0091-6749 (Linking)
Abstract:		"BACKGROUND: Domestic mammals are important sources of indoor allergens. However, the origin at the tissue level and the biologic function of mammalian allergens are largely unknown. OBJECTIVE: The aim of this study was to localize the source of the major bovine dander allergen, Bos d 2, in bovine tissues. METHODS: Samples from several organs were tested for the presence of mRNA encoding Bos d 2 and Bos d 2 protein by using the reverse transcriptase polymerase chain reaction and immunohistochemical staining, respectively. RESULTS: Skin proved to be the only tissue where mRNA encoding Bos d 2 was detected. This observation was confirmed by immunohistochemistry with a monoclonal anti-Bos d 2 antibody as the primary antibody. In the skin sections, Bos d 2 was found in the secretory cells of apocrine sweat glands and the basement membranes of the epithelium and hair follicles. Bos d 2 belongs to the family of lipocalins comprising a number of pheromone carrier proteins that are present, for example, in the secretions of the apocrine sweat glands. CONCLUSION: Together with earlier data, our findings suggest that Bos d 2 is produced in sweat glands and transported to the skin surface as a carrier of the pheromone ligand. Because dander allergens of a number of mammalian species are lipocalins, the common biologic function of being pheromone carriers seems to be a common feature of an important group of aeroallergens"
Keywords:		"Allergens/genetics/*isolation & purification/metabolism Animals Antigens, Plant Cattle Epithelium/metabolism Female Hair Follicle/metabolism Hypersensitivity, Immediate/*immunology Immunohistochemistry Kidney/metabolism Liver/metabolism Male Mammary Gland;"
Notes:		"MedlineRautiainen, J Rytkonen, M Syrjanen, K Pentikainen, J Zeiler, T Virtanen, T Mantyjarvi, R eng Research Support, Non-U.S. Gov't 1998/04/03 J Allergy Clin Immunol. 1998 Mar; 101(3):349-53. doi: 10.1016/S0091-6749(98)70247-7"