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J Chromatogr A

Title:		Single-injection calibration approach for high-performance liquid chromatography
Author(s):		Paredes E; Maestre SE; Prats MS; Todoli JL;
Address:		"Department of Analytical Chemistry, Nutrition and Food Sciences, University of Alicante, 03080 Alicante, Spain"
Journal Title:		J Chromatogr A
Year:		2008
Volume:		20080131
Issue:		2
Page Number:		178 - 184
DOI:		10.1016/j.chroma.2008.01.071
ISSN/ISBN:		0021-9673 (Print) 0021-9673 (Linking)
Abstract:		"A new calibration method for high-performance liquid chromatography was validated. The method was called single-injection calibration approach (SICA) because it allowed to obtain a complete calibration curve by means of a single injection of a standard solution containing several non-volatile and semi-volatile organic compounds at different concentration levels. The compounds studied included carboxylic acids, polyalcohols, carbohydrates and water-soluble vitamins. This method allowed a 1-7-fold reduction in the analysis time with regard to conventional calibration methods. The method was applied to three different chromatographic detection methods: refractive index (RI) detection, diode array detection (DAD) and inductively coupled plasma atomic emission detection (ICP-AED). Good linearity was achieved (r(2)>0.999) for the three detection methods but signal correction was required for RI detection and DAD. This fact demonstrated that ICP-AES was the most universal because the signal obtained for non-volatile and semi-volatile organic compounds was not a function of the chemical nature of the compound and only depended on the mass content of carbon. The method was validated by analyzing a reference non-fat milk powder sample as well as several real food samples (three fruit juices, four wines, three candies and a multivitamin complex)"
Keywords:		"Calibration Chromatography, High Pressure Liquid/*methods Food Analysis/*methods Organic Chemicals/*analysis;"
Notes:		"MedlineParedes, Eduardo Maestre, Salvador E Prats, Ma Soledad Todoli, Jose L eng Research Support, Non-U.S. Gov't Validation Study Netherlands 2008/02/27 J Chromatogr A. 2008 Mar 28; 1185(2):178-84. doi: 10.1016/j.chroma.2008.01.071. Epub 2008 Jan 31"