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J Biol Chem

Title:		"Specific induction of Ca2+ transport activity in MATa cells of Saccharomyces cerevisiae by a mating pheromone, alpha factor"
Author(s):		Ohsumi Y; Anraku Y;
Address:
Journal Title:		J Biol Chem
Year:		1985
Volume:		260
Issue:		19
Page Number:		10482 - 10486
DOI:
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"Incubation with a high concentration of a mating pheromone, alpha factor, of Saccharomyces cerevisiae induced the accumulation of Ca2+ ion in MATa cells, but not in MAT alpha or MATa/alpha cells, after a lag of 30-40 min. The alpha factor did not cause a nonspecific lesion of the membrane barrier, but induced Ca2+ transport activity specifically. This induction of Ca2+ transport activity correlated with formation of a projection on the cells, or with localized cell elongation, but not with G1 arrest or agglutinin induction. The increased Ca2+ transport activity was maintained only in the continuous presence of a high concentration of alpha factor and de novo protein synthesis. Kinetic studies of induction of Ca2+ transport by alpha factor and effects of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid and Ca2+ ionophore, A23187, on mating suggested an essential role of this physiological reaction in the initiation of sexual conjugation"
Keywords:		Animals Calcium/*pharmacology Calcium-Transporting ATPases/*biosynthesis Cycloheximide/pharmacology Enzyme Induction Kinetics Mating Factor Peptides/*physiology Pheromones/*physiology Saccharomyces cerevisiae/drug effects/*metabolism Species Specificity;
Notes:		"MedlineOhsumi, Y Anraku, Y eng Research Support, Non-U.S. Gov't 1985/09/05 J Biol Chem. 1985 Sep 5; 260(19):10482-6"