| Title: | UGA is translated as cysteine in pheromone 3 of Euplotes octocarinatus |
| Author(s): | Meyer F; Schmidt HJ; Plumper E; Hasilik A; Mersmann G; Meyer HE; Engstrom A; Heckmann K; |
| Address: | "Institute of Zoology, University of Munster, Federal Republic of Germany" |
| ISSN/ISBN: | 0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking) |
| Abstract: | "Pheromone 3 mRNA of the ciliate Euplotes octocarinatus contains three in-frame UGA codons that are translated as cysteines. This was revealed from cDNA sequencing and from plasma desorption mass spectrometry of cleaved pheromone 3 in connection with pyridylethylation of the fragments. N-terminal sequence analysis of carboxymethylated protein confirmed this conclusion for the first of the three UGA codons. Besides UGA the common cysteine codons UGU and UGC are also used to encode cysteine. UAA functions as a termination codon. No UAG codon was found. In connection with results reported for other ciliates, this suggests that the role of the classic termination codons had not yet been established when the ciliates started to diverge from other eukaryotes" |
| Keywords: | "Amino Acid Sequence Animals Base Sequence Ciliophora/*genetics/physiology *Cysteine DNA/genetics *Genetic Code Mass Spectrometry Molecular Sequence Data *Peptide Chain Termination, Translational Peptide Fragments/chemistry Pheromones/*genetics Restriction;" |
| Notes: | "MedlineMeyer, F Schmidt, H J Plumper, E Hasilik, A Mersmann, G Meyer, H E Engstrom, A Heckmann, K eng Research Support, Non-U.S. Gov't 1991/05/01 Proc Natl Acad Sci U S A. 1991 May 1; 88(9):3758-61. doi: 10.1073/pnas.88.9.3758" |
