| Title: | Pheromone Perception: Mechanism of the Reversible Coil-Helix Transition in Antheraea polyphemus Pheromone-Binding Protein 1 |
| Author(s): | Mazumder S; Chaudhary BP; Dahal SR; Al-Danoon O; Mohanty S; |
| Address: | "Department of Chemistry , Oklahoma State University , Stillwater , Oklahoma 74078 , United States" |
| DOI: | 10.1021/acs.biochem.9b00737 |
| ISSN/ISBN: | 1520-4995 (Electronic) 0006-2960 (Linking) |
| Abstract: | "Pheromone-binding protein (PBP) in male moth antennae transports pheromone to the olfactory receptor neuron by undergoing a pH-dependent conformational switch, from PBP(B) at higher pH to PBP(A) at lower pH, associated with ligand binding and release, respectively. The characteristic feature of the dramatic protein switch is the pH-dependent reversible coil-helix transition of the C-terminus. In the PBP(B) conformation at pH >6.0, the C-terminus is exposed to the solvent as a coil while the ligand occupies the hydrophobic pocket. However, in the PBP(A) conformation at acidic pH, the C-terminus switches to a helix and releases the ligand by outcompeting it for the hydrophobic pocket. In Antheraea polyphemus PBP1 (ApolPBP1), the C-terminus (P(129)-V(142)) is composed predominantly of hydrophobic residues except for three strategically located acidic residues: Asp(132), Glu(137), and Glu(141). Here, we report for the first time on the consequences of the mutation of one or more acidic residues in the pH-driven reversible coil-helix transition of the ApolPBP1 C-terminus through biophysical characterization. Mutation of any single acidic residue in the C-terminus to its neutral counterpart destabilizes the helix formation at lower pH; these mutants exist as a mixture of both conformations. However, mutation of the two terminal acidic residues together knocks out the protein switch and adversely affects both ligand binding and release functions. Thus, these mutant proteins remain in the open (PBP(B)) conformation at all pH levels" |
| Keywords: | "Amino Acid Sequence Animals Hydrogen-Ion Concentration Insect Proteins/chemistry/*metabolism Male Models, Molecular Moths/chemistry/*physiology Pheromones/*metabolism Protein Conformation Protein Conformation, alpha-Helical Sequence Alignment;" |
| Notes: | "MedlineMazumder, Suman Chaudhary, Bharat P Dahal, Salik R Al-Danoon, Omar Mohanty, Smita eng Research Support, U.S. Gov't, Non-P.H.S. 2019/10/23 Biochemistry. 2019 Nov 12; 58(45):4530-4542. doi: 10.1021/acs.biochem.9b00737. Epub 2019 Nov 1" |
