« Previous AbstractOral cues are not enough: induction of defensive proteins in Nicotiana tabacum upon feeding by caterpillars    Next Abstract"Stomata-mediated interactions between plants, herbivores, and the environment" »

New Phytol

Title:		Silencing the alarm: an insect salivary enzyme closes plant stomata and inhibits volatile release
Author(s):		Lin PA; Chen Y; Chaverra-Rodriguez D; Heu CC; Zainuddin NB; Sidhu JS; Peiffer M; Tan CW; Helms A; Kim D; Ali J; Rasgon JL; Lynch J; Anderson CT; Felton GW;
Address:		"Department of Entomology, Pennsylvania State University, 501 ASI Building, University Park, PA, 16802, USA. Department of Biology, Pennsylvania State University, 415 Life Sciences Building, University Park, PA, 16802, USA. Department of Cell and Developmental Biology, University of California San Diego, 9500 Gilman Drive #0335, La Jolla, CA, 92093, USA. Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, Serdang, Selangor, 43400 UPM, Malaysia. Department of Plant Science, Pennsylvania State University, 310 Tyson Building, University Park, PA, 16802, USA. Department of Entomology, 103DA Entomology Research Laboratory, Texas A&M University, College Station, TX, 77843, USA. Department of Applied Biology, Kyungpook National University, 80 Daehakro, Bukgu, Daegu, 41566, Korea"
Journal Title:		New Phytol
Year:		2021
Volume:		20210216
Issue:		2
Page Number:		793 - 803
DOI:		10.1111/nph.17214
ISSN/ISBN:		1469-8137 (Electronic) 0028-646X (Print) 0028-646X (Linking)
Abstract:		"Herbivore-induced plant volatiles (HIPVs) are widely recognized as an ecologically important defensive response of plants against herbivory. Although the induction of this 'cry for help' has been well documented, only a few studies have investigated the inhibition of HIPVs by herbivores and little is known about whether herbivores have evolved mechanisms to inhibit the release of HIPVs. To examine the role of herbivore effectors in modulating HIPVs and stomatal dynamics, we conducted series of experiments combining pharmacological, surgical, genetic (CRISPR-Cas9) and chemical (GC-MS analysis) approaches. We show that the salivary enzyme, glucose oxidase (GOX), secreted by the caterpillar Helicoverpa zea on leaves, causes stomatal closure in tomato (Solanum lycopersicum) within 5 min, and in both tomato and soybean (Glycine max) for at least 48 h. GOX also inhibits the emission of several HIPVs during feeding by H. zea, including (Z)-3-hexenol, (Z)-jasmone and (Z)-3-hexenyl acetate, which are important airborne signals in plant defenses. Our findings highlight a potential adaptive strategy where an insect herbivore inhibits plant airborne defenses during feeding by exploiting the association between stomatal dynamics and HIPV emission"
Keywords:		Animals Herbivory Insecta *Moths Plant Stomata *Volatile Organic Compounds Hipv effector insect herbivore plant defense stomata;
Notes:		"MedlineLin, Po-An Chen, Yintong Chaverra-Rodriguez, Duverney Heu, Chan Chin Zainuddin, Nursyafiqi Bin Sidhu, Jagdeep Singh Peiffer, Michelle Tan, Ching-Wen Helms, Anjel Kim, Donghun Ali, Jared Rasgon, Jason L Lynch, Jonathan Anderson, Charles T Felton, Gary W eng Research Support, U.S. Gov't, Non-P.H.S. England 2021/01/19 New Phytol. 2021 Apr; 230(2):793-803. doi: 10.1111/nph.17214. Epub 2021 Feb 16"