| Title: | Interaction among Saccharomyces cerevisiae pheromone receptors during endocytosis |
| Author(s): | Chang CI; Schandel KA; Jenness DD; |
| Address: | "Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, 55 Lake Avenue North, Worcester, MA 01655, USA" |
| ISSN/ISBN: | 2046-6390 (Print) 2046-6390 (Electronic) 2046-6390 (Linking) |
| Abstract: | "This study investigates endocytosis of Saccharomyces cerevisiae alpha-factor receptor and the role that receptor oligomerization plays in this process. alpha-factor receptor contains signal sequences in the cytoplasmic C-terminal domain that are essential for ligand-mediated endocytosis. In an endocytosis complementation assay, we found that oligomeric complexes of the receptor undergo ligand-mediated endocytosis when the alpha-factor binding site and the endocytosis signal sequences are located in different receptors. Both in vitro and in vivo assays suggested that ligand-induced conformational changes in one Ste2 subunit do not affect neighboring subunits. Therefore, recognition of the endocytosis signal sequence and recognition of the ligand-induced conformational change are likely to be two independent events" |
| Keywords: | G-protein coupled receptors Ligand-mediated endocytosis Receptor oligomers; |
| Notes: | "PubMed-not-MEDLINEChang, Chien-I Schandel, Kimberly A Jenness, Duane D eng England 2014/04/01 Biol Open. 2014 Apr 15; 3(4):297-306. doi: 10.1242/bio.20146866" |
