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Sci Rep

Title:		Oxidative damage and impairment of protein quality control systems in keratinocytes exposed to a volatile organic compounds cocktail
Author(s):		Dezest M; Le Bechec M; Chavatte L; Desauziers V; Chaput B; Grolleau JL; Descargues P; Nizard C; Schnebert S; Lacombe S; Bulteau AL;
Address:		"IPREM, UMR 5254, Universite de Pau et des Pays de l'Adour, Helioparc, 2 Avenue Pierre Angot, 64000, Pau, France. Pole RIME-C2MA, Ecole des mines d'Ales, Helioparc, 2 Avenue Pierre Angot, 64053, Pau, France. Service de chirurgie plastique et reconstructrice, CHU de Toulouse Rangueil, 1, avenue Jean-Poulhes, 31400, Toulouse, France. Genoskin, Centre Pierre Potier, Oncopole, 31100, Toulouse, France. LVMH Recherche. Life Science Department, 185 Avenue de Verdun, 45800, Saint Jean de Braye, France. IPREM, UMR 5254, Universite de Pau et des Pays de l'Adour, Helioparc, 2 Avenue Pierre Angot, 64000, Pau, France. anne-laure.bulteau@ens-lyon.fr. Institut de Genomique Fonctionnelle de Lyon (IGFL), Ecole Normale Superieure (ENS) de Lyon-CNRS- UMR 5242, 46 Allee d'Italie, 69364 Lyon, France. anne-laure.bulteau@ens-lyon.fr"
Journal Title:		Sci Rep
Year:		2017
Volume:		20170906
Issue:		1
Page Number:		10707 -
DOI:		10.1038/s41598-017-11088-1
ISSN/ISBN:		2045-2322 (Electronic) 2045-2322 (Linking)
Abstract:		"Compelling evidence suggests that volatile organic compounds (VOCs) have potentially harmful effects to the skin. However, knowledge about cellular signaling events and toxicity subsequent to VOC exposure to human skin cells is still poorly documented. The aim of this study was to focus on the interaction between 5 different VOCs (hexane, toluene, acetaldehyde, formaldehyde and acetone) at doses mimicking chronic low level environmental exposure and the effect on human keratinocytes to get better insight into VOC-cell interactions. We provide evidence that the proteasome, a major intracellular proteolytic system which is involved in a broad array of processes such as cell cycle, apoptosis, transcription, DNA repair, protein quality control and antigen presentation, is a VOC target. Proteasome inactivation after VOC exposure is accompanied by apoptosis, DNA damage and protein oxidation. Lon protease, which degrades oxidized, dysfunctional, and misfolded proteins in the mitochondria is also a VOC target. Using human skin explants we found that VOCs prevent cell proliferation and also inhibit proteasome activity in vivo. Taken together, our findings provide insight into potential mechanisms of VOC-induced proteasome inactivation and the cellular consequences of these events"
Keywords:		"Apoptosis/drug effects Biomarkers DNA Damage Glutathione/metabolism Humans Immunophenotyping Keratinocytes/*drug effects/*metabolism Oxidation-Reduction *Oxidative Stress Proteasome Endopeptidase Complex/metabolism Protein Processing, Post-Translational/*;"
Notes:		"MedlineDezest, Marlene Le Bechec, Mickael Chavatte, Laurent Desauziers, Valerie Chaput, Benoit Grolleau, Jean-Louis Descargues, Pascal Nizard, Carine Schnebert, Sylvianne Lacombe, Sylvie Bulteau, Anne-Laure eng Research Support, Non-U.S. Gov't England 2017/09/08 Sci Rep. 2017 Sep 6; 7(1):10707. doi: 10.1038/s41598-017-11088-1"