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Curr Biol

Title:		Prostaglandin E(2) synchronizes lunar-regulated beach spawning in grass puffers
Author(s):		Chen J; Katada Y; Okimura K; Yamaguchi T; Guh YJ; Nakayama T; Maruyama M; Furukawa Y; Nakane Y; Yamamoto N; Sato Y; Ando H; Sugimura A; Tabata K; Sato A; Yoshimura T;
Address:		"Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan; Laboratory of Animal Integrative Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan. Laboratory of Animal Integrative Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan; Institute for Advanced Research, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan. Laboratory of Fish Biology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan. Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan. Sado Marine Biological Station, Sado Island Center for Ecological Sustainability, Niigata University, 87 Tassha, Sado 952-2135, Niigata, Japan. Toyota Boshoku Corporation, 1-1 Toyoda-cho, Kariya 448-8651, Aichi, Japan. Institute of Transformative Bio-Molecules (WPI-ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan; Laboratory of Animal Integrative Physiology, Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Aichi, Japan. Electronic address: takashiy@agr.nagoya-u.ac.jp"
Journal Title:		Curr Biol
Year:		2022
Volume:		20221027
Issue:		22
Page Number:		4881 - 4889
DOI:		10.1016/j.cub.2022.09.062
ISSN/ISBN:		1879-0445 (Electronic) 0960-9822 (Linking)
Abstract:		"Many organisms living along the coastlines synchronize their reproduction with the lunar cycle. At the time of spring tide, thousands of grass puffers (Takifugu alboplumbeus) aggregate and vigorously tremble their bodies at the water's edge to spawn. To understand the mechanisms underlying this spectacular semilunar beach spawning, we collected the hypothalamus and pituitary from male grass puffers every week for 2 months. RNA sequencing (RNA-seq) analysis identified 125 semilunar genes, including genes crucial for reproduction (e.g., gonadotropin-releasing hormone 1 [gnrh1], luteinizing hormone beta subunit [lhb]) and receptors for pheromone prostaglandin E (PGE). PGE(2) is secreted into the seawater during the spawning, and its administration activates olfactory sensory neurons and triggers trembling behavior of surrounding individuals. These results suggest that PGE(2) synchronizes lunar-regulated beach-spawning behavior in grass puffers. To further explore the mechanism that regulates the lunar-synchronized transcription of semilunar genes, we searched for semilunar transcription factors. Spatial transcriptomics and multiplex fluorescent in situ hybridization showed co-localization of the semilunar transcription factor CCAAT/enhancer-binding protein delta (cebpd) and gnrh1, and cebpd induced the promoter activity of gnrh1. Taken together, our study demonstrates semilunar genes that mediate lunar-synchronized beach-spawning behavior. VIDEO ABSTRACT"
Keywords:		"Humans Animals Male *Takifugu/genetics/metabolism *Moon In Situ Hybridization, Fluorescence Reproduction/physiology Prostaglandins E/metabolism Prostaglandins/metabolism beach spawning biological clock circalunar rhythms grass puffer lunar cycle neap tide;"
Notes:		"MedlineChen, Junfeng Katada, Yuma Okimura, Kousuke Yamaguchi, Taiki Guh, Ying-Jey Nakayama, Tomoya Maruyama, Michiyo Furukawa, Yuko Nakane, Yusuke Yamamoto, Naoyuki Sato, Yoshikatsu Ando, Hironori Sugimura, Asako Tabata, Kazufumi Sato, Ayato Yoshimura, Takashi eng Research Support, Non-U.S. Gov't Video-Audio Media England 2022/10/29 Curr Biol. 2022 Nov 21; 32(22):4881-4889.e5. doi: 10.1016/j.cub.2022.09.062. Epub 2022 Oct 27"