| Title: | Broadly Tuned Odorant Receptor AlinOR59 Involved in Chemoreception of Floral Scent in Adelphocoris lineolatus |
| Author(s): | Xiao Y; An XK; Khashaveh A; Shan S; Wang Q; Wang SN; Gu SH; Li ZY; Zhang YJ; |
| Address: | "Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China. College of Plant Protection, China Agricultural University, Beijing 100193, China" |
| ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
| Abstract: | "Plant volatiles such as floral scent compounds play a crucial role in mediating insect host locating, mate search, and oviposition sites selection. The alfalfa plant bug, Adelphocoris lineolatus (Goeze), is a seriously polyphagous herbivore of alfalfa and cotton that has an obvious preference for flowering host plants. In this study, we focused on the role of an odorant receptor AlinOR59 in the perception of plant volatiles in A. lineolatus. In situ hybridization showed that AlinOR59 was coexpressed with the coreceptor AlinORco in the ORNs cell located in the long curved sensilla trichodea on antennae of both genders. The Xenopus oocytes expression coupled with two-electrode voltage clamp recordings demonstrated that AlinOR59 responded to 15 plant volatiles. In electroantennogram assays, all of the above 15 compounds could excite electrophysiological responses in the antennae of adult bugs. Furthermore, an important floral scent compound, methyl salicylate, was utilized to evaluate the behavioral responses of A. lineolatus. It was found that adult bugs of both genders were significantly attracted to methyl salicylate. Taken together, our findings suggest that AlinOR59 plays a crucial role in the perception of floral scents in A. lineolatus and could be used as a potential target to design novel olfactory regulators for the management of bugs" |
| Keywords: | "Animals Arthropod Antennae Female Flowers/chemistry *Heteroptera Insect Proteins/genetics Male Odorants *Receptors, Odorant/genetics Sensilla Adelphocoris lineolatus behavioral response floral scents methyl salicylate odorant receptor AlinOR59 two-electro;" |
| Notes: | "MedlineXiao, Yong An, Xing-Kui Khashaveh, Adel Shan, Shuang Wang, Qi Wang, Shan-Ning Gu, Shao-Hua Li, Zhen-Yu Zhang, Yong-Jun eng 2020/11/06 J Agric Food Chem. 2020 Nov 25; 68(47):13815-13823. doi: 10.1021/acs.jafc.0c04434. Epub 2020 Nov 5" |
