| Title: | The application of rapid evaporative ionization mass spectrometry in the analysis of Drosophila species-a potential new tool in entomology |
| Author(s): | Wagner I; Koch NI; Sarsby J; White N; Price TAR; Jones S; Hurst JL; Beynon RJ; |
| Address: | "Centre for Proteome Research, Institute of Systems, Molecular and Integrative Biology, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK. Ecology and Evolution Group, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Crown Street, Liverpool L69 7ZB, UK. International Pheromone Systems Ltd, Unit 8, West Float Industrial Estate, Millbrook Road, Wallasey, Wirral CH41 1FL, UK. Mammalian Behaviour and Evolution Group, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Leahurst Campus, Neston CH64 7TE, UK" |
| ISSN/ISBN: | 2046-2441 (Electronic) 2046-2441 (Linking) |
| Abstract: | "There is increasing emphasis on the use of new analytical approaches in subject analysis and classification, particularly in respect to minimal sample preparation. Here, we demonstrate that rapid evaporative ionization mass spectrometry (REIMS), a method that captures metabolite mass spectra after rapid combustive degradation of an intact biological specimen, generates informative mass spectra from several arthropods, and more specifically, is capable of discerning differences between species and sex of several adult Drosophila species. A model including five Drosophila species, built using pattern recognition, achieves high correct classification rates (over 90%) using test datasets and is able to resolve closely related species. The ease of discrimination of male and female specimens also demonstrates that sex-specific differences reside in the REIMS metabolite patterns, whether analysed across all five species or specifically for D. melanogaster. Further, the same approach can correctly discriminate and assign Drosophila species at the larval stage, where these are morphologically highly similar or identical. REIMS offers a novel approach to insect typing and analysis, requiring a few seconds of data acquisition per sample and has considerable potential as a new tool for the field biologist" |
| Keywords: | Animals Data Analysis Drosophila/*classification Female Male Mass Spectrometry/*methods Species Specificity Drosophila Reims insects mass spectrometry species identification; |
| Notes: | "MedlineWagner, Iris Koch, Natalie I Sarsby, Joscelyn White, Nicola Price, Tom A R Jones, Sam Hurst, Jane L Beynon, Robert J eng BB/L014793/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom Research Support, Non-U.S. Gov't England 2020/11/26 Open Biol. 2020 Nov; 10(11):200196. doi: 10.1098/rsob.200196. Epub 2020 Nov 25" |
