« Previous AbstractAlteration of kairomone-induced antipredator response of the freshwater amphipod Gammarus roeseli by sediment type    Next AbstractA methodology for ranking and hazard identification of xenobiotic organic compounds in urban stormwater »

Archaea

Title:		Molybdate treatment and sulfate starvation decrease ATP and DNA levels in Ferroplasma acidarmanu
Author(s):		Baumler DJ; Hung KF; Jeong KC; Kaspar CW;
Address:		"Cellular and Molecular Biology, University of Wisconsin, Madison, Wisconsin, USA"
Journal Title:		Archaea
Year:		2008
Volume:		2
Issue:		3
Page Number:		205 - 209
DOI:		10.1155/2008/762967
ISSN/ISBN:		1472-3646 (Print) 1472-3654 (Electronic) 1472-3646 (Linking)
Abstract:		"Sulfate is a primary source of sulfur for most microbes and in some prokaryotes it is used an electron acceptor. The acidophile Ferroplasma acidarmanus (strain fer1) requires a minimum of 150 mM of a sulfate-containing salt for growth. Sulfate is assimilated by F. acidarmanus into proteins and reduced to form the volatile organic sulfur compounds methanethiol and dimethyldisulfide. In the absence of sulfate, cell death occurs by an unknown mechanism. In this study, cell viability and genomic DNA and ATP contents of F. acidarmanus were monitored in response to the absence of sulfate or the presence of sulfate and the sulfate analog molybdate (MoO(4) (2-)). Cellular DNA and ATP contents were monitored as markers of cell viability. The absence of sulfate led to a decrease in viable cell numbers of greater than 7 log(10 )within 5 days, a > 99% reduction in genomic DNA within 3 days, and a > 60% decrease in ATP within 6 h. Likewise, cells incubated with MoO(4) (2-) lost viability (decreased by > 2 log(10) in 5 days), extractable genomic DNA (reduction of > 60% in 2 days), and ATP (reduction of > 70 % in 2 hours). These results demonstrate that sulfate deprivation or the presence of molybdate have similar impacts on cell viability and essential biomolecules. Sulfate was coupled to cellular ATP content and maintenance of DNA integrity in F. acidarmanus, a finding that may be applicable to other acidophiles that are typically found in sulfate-rich biotopes"
Keywords:		"Adenosine Triphosphate/*metabolism Archaeal Proteins/genetics/metabolism Colony Count, Microbial Culture Media DNA, Archaeal/genetics/*metabolism Gene Expression Regulation, Archaeal Homeostasis Hydrogen-Ion Concentration Molybdenum/*metabolism Sulfates/*;"
Notes:		"MedlineBaumler, David J Hung, Kai-Foong Jeong, Kwang Cheol Kaspar, Charles W eng Research Support, Non-U.S. Gov't 2008/12/05 Archaea. 2008 Dec; 2(3):205-9. doi: 10.1155/2008/762967"