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J Anal Toxicol

Title:		Determination of volatile organic compounds in biological samples using headspace solid-phase microextraction and gas chromatography: toluene and styrene
Author(s):		Barua R; Chi LH; Fitzpatrick R; Gillard D; Kostyniak PJ;
Address:		"Department of Clinical and Laboratory Sciences, SUNY at Buffalo, Buffalo, New York, USA"
Journal Title:		J Anal Toxicol
Year:		2008
Volume:		32
Issue:		5
Page Number:		379 - 386
DOI:		10.1093/jat/32.5.379
ISSN/ISBN:		0146-4760 (Print) 0146-4760 (Linking)
Abstract:		"Epidemiological and laboratory investigations have shown that toluene and styrene are toxic compounds that lead to impairment of the nervous system. To quantitate toluene and styrene in biological samples, liquid-liquid phase, headspace (HS), and solid-phase microextraction (SPME) methods are generally used. Most of these methods are not sensitive enough for applications involving small sample volumes. Here, we present a method for quantitative analysis of low concentrations of styrene and toluene in very small volumes of biological samples using HS-SPME and gas chromatography (GC) equipped with a flame-ionization detector. The method was developed by optimizing operating parameters that affect the HS-SPME-GC process [i.e., desorption time (30 s), depth of the fiber in the GC injection port (3.7 cm), adsorption time (4 min), and adsorption temperature (room temperature)]. It has a wide range of linearity (0.5-500 ng/10 microL), high precision (coefficient of variation < 5%), good accuracy (deviation < 11%), and low detection limits of 0.13 and 0.08 ng/10 microL for styrene and toluene in serum, respectively. This analytical technique can be applied to the estimation of styrene and toluene in small volumes of biological fluids (blood, serum, and perilymph) and tissues of low lipid content (cochlea)"
Keywords:		"Air Pollutants, Occupational/*blood Chromatography, Gas/methods Environmental Monitoring/*methods Humans Solid Phase Extraction/*methods Solvents/*metabolism Styrene/*blood Toluene/*blood Volatilization;"
Notes:		"MedlineBarua, Radhika Chi, Lai-Har Fitzpatrick, Richard Gillard, Douglas Kostyniak, Paul J eng R8113-01A1OSH/PHS HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Validation Study England 2008/06/12 J Anal Toxicol. 2008 Jun; 32(5):379-86. doi: 10.1093/jat/32.5.379"