| Title: | Human-like smelling of a rose scent using an olfactory receptor nanodisc-based bioelectronic nose |
| Author(s): | Lee M; Yang H; Kim D; Yang M; Park TH; Hong S; |
| Address: | "Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul, 08826, Korea. School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Korea. Protein Engineering Laboratory, Recombinants Unit, MOGAM Institute for Biomedical Research, Yongin, 16924, Korea. Department of Biophysics and Chemical Biology, Seoul National University, Seoul, 08826, Korea. School of Chemical and Biological Engineering, Seoul National University, Seoul, 08826, Korea. thpark@snu.ac.kr. Department of Physics and Astronomy and Institute of Applied Physics, Seoul National University, Seoul, 08826, Korea. seunghun@snu.ac.kr" |
| DOI: | 10.1038/s41598-018-32155-1 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "We report a strategy for the human-like smelling of a rose scent utilizing olfactory receptor nanodisc (ND)-based bioelectronic nose devices. In this strategy, a floating electrode (FE)-based carbon nanotube (CNT) field effect transistor (FET) was functionalized with human olfactory receptor 1A2 (hOR1A2)-embedded NDs (hOR1A2NDs). The hOR1A2NDs responded to rose scent molecules specifically, which were monitored electrically using the underlying CNT-FET. This strategy allowed us to quantitatively assess the contents of geraniol and citronellol, the main components of a rose scent, as low as 1 fM and 10 fM, respectively. In addition, it enabled us to selectively discriminate a specific rose odorant from other odorants. Significantly, we also demonstrated that the responses of hOR1A2NDs to a rose scent could be strongly enhanced by enhancer materials like a human nose. Furthermore, the method provided a means to quantitatively evaluate rose scent components in real samples such as rose oil. Since our method allows one to quantitatively evaluate general rose scent ingredients just like a human nose, it could be a powerful strategy for versatile basic research and various applications such as fragrance development" |
| Keywords: | "Biosensing Techniques/*methods Electronic Nose Humans Nose/*physiology Odorants Olfactory Receptor Neurons/metabolism Pheromones/metabolism Receptors, Odorant/*metabolism Rosa/*metabolism Smell/*physiology Transistors, Electronic;" |
| Notes: | "MedlineLee, Minju Yang, Heehong Kim, Daesan Yang, Myungjae Park, Tai Hyun Hong, Seunghun eng Research Support, Non-U.S. Gov't England 2018/09/19 Sci Rep. 2018 Sep 17; 8(1):13945. doi: 10.1038/s41598-018-32155-1" |
