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Arch Microbiol

Title:		"Genomic and metabolic features of Bacillus cereus, inhibiting the growth of Sclerotinia sclerotiorum by synthesizing secondary metabolites"
Author(s):		Hu J; Dong B; Wang D; Meng H; Li X; Zhou H;
Address:		"College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010020, Inner Mongolia, China. Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology Center, Hohhot, 010020, Inner Mongolia, China. College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot, 010020, Inner Mongolia, China. hongyouzhou2002@aliyun.com. Inner Mongolia Cold and Arid Region Crop Protection Engineering Technology Center, Hohhot, 010020, Inner Mongolia, China. hongyouzhou2002@aliyun.com"
Journal Title:		Arch Microbiol
Year:		2022
Volume:		20221201
Issue:		1
Page Number:		8 -
DOI:		10.1007/s00203-022-03351-5
ISSN/ISBN:		1432-072X (Electronic) 0302-8933 (Print) 0302-8933 (Linking)
Abstract:		"We investigated the biocontrol mechanism of Bacillus cereus CF4-51 to find powerful microbes that effectively control Sclerotinia sclerotiorum. To assess its inhibitory effect on fungal growth, the plant pathogen (S. sclerotiorum) was co-cultured with Bacillus cereus. Scanning electron microscope (SEM) was used to study the morphology of S. sclerotiorum treated with CF4-51 biofumigant. The expression of sclerotium formation-related genes was analyzed by qRT-PCR. We performed whole genome sequencing of CF4-51 by PacBio Sequel platform. Lipopeptides were extracted from strain CF4-51 according to the method of hydrochloric acid precipitation and methanol dissolution. The volatiles CF4-51 were identified using gas chromatography-mass spectrometry (GC-MS). We found that the volatile organic compounds (VOCs) released by CF4-51 damaged the S. sclerotiorum hyphae and inhibited the formation of sclerotia. The qRT-PCR data revealed the down-regulated expression of the genes involved in sclerotial formation. Moreover, we analyzed the B. cereus CF4-51 genome and metabolites. The genome consisted of 5.35 Mb, with a GC content of 35.74%. An examination of the genome revealed the presence of several gene clusters for the biosynthesis of antibiotics, siderophores, and various other bioactive compounds, including those belonging to the NRPS-like, LAP, RIPP-like, NRPS, betalactone, CDPS, terpene, ladderane, ranthipeptide, and lanthipeptide (class II) categories. A gas chromatography-tandem mass spectrometry analysis identified 45 VOCs produced by strain CF4-51. Among these, technical grade formulations of five were chosen for further study: 2-Pentadecanone, 6,10,14-trimethyl-,1,2-Benzenedicarboxylic acid, bis(2-methylpropyl) ester, Dibutyl phthalate, Cyclododecane, Heptadecane. the five major constituents play important roles in the antifungal activity of the VOCs CF4-51 on the growth of S. sclerotiorum. The secondary metabolites produced by strain CF4-51are critical for the inhibition of S. sclerotiorum hyphal growth and sclerotial formation"
Keywords:		Bacillus cereus/genetics Gas Chromatography-Mass Spectrometry Genomics *Ascomycota/genetics *Volatile Organic Compounds Antifungal activity Bacillus cereus Genome mining Sclerotinia sclerotiorum Secondary metabolites;
Notes:		"MedlineHu, Jinghan Dong, Baozhu Wang, Dong Meng, Huanwen Li, Xiaojuan Zhou, Hongyou eng 2017YFD0201101/the earmarked fund for National Key R&D Projects/ 31260445/the National Natural Science Foundation of China/ Germany 2022/12/02 Arch Microbiol. 2022 Dec 1; 205(1):8. doi: 10.1007/s00203-022-03351-5"