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Anal Chim Acta

Title:		Automated SPME-GC-MS monitoring of headspace metabolomic responses of E. coli to biologically active components extracted by the coating
Author(s):		Hossain SM; Bojko B; Pawliszyn J;
Address:		"Department of Chemistry, University of Waterloo, Waterloo, ON, Canada"
Journal Title:		Anal Chim Acta
Year:		2013
Volume:		20130315
Issue:
Page Number:		41 - 49
DOI:		10.1016/j.aca.2013.03.018
ISSN/ISBN:		1873-4324 (Electronic) 0003-2670 (Linking)
Abstract:		"Monitoring extracellular metabolites of bacteria is very useful for not only metabolomics research but also for assessment of the effects of various chemicals, including antimicrobial agents and drugs. Herein, we describe the automated headspace solid-phase microextraction (HS-SPME) method coupled with gas chromatography-mass spectrometry (GC-MS) for the qualitative as well as semi-quantitative determination of metabolic responses of Escherichia coli to an antimicrobial agent, cinnamaldehyde. The minimum inhibitory concentration of cinnamaldehyde was calculated to be 2 g L(-1). We found that cinnamaldehyde was an important factor influencing the metabolic profile and growth process. A higher number of metabolites were observed during the mid-logarithmic growth phase. The metabolite variations (types and concentrations) induced by cinnamaldehyde were dependent on both cell density and the dose of cinnamaldehyde. Simultaneously, 25 different metabolites were separated and detected (e.g., indole, alkane, alcohol, organic acids, esters, etc.) in headspace of complex biological samples due to intermittent addition of high dose of cinnamaldehyde. The study was done using an automated system, thereby minimizing manual workup and indicating the potential of the method for high-throughput analysis. These findings enhanced the understanding of the metabolic responses of E. coli to cinnamaldehyde shock effect and demonstrated the effectiveness of the SPME-GC-MS based metabolomics approach to study such a complex biological system"
Keywords:		Acrolein/analogs & derivatives/pharmacology Automation Escherichia coli/drug effects/*metabolism *Gas Chromatography-Mass Spectrometry *Metabolome Microbial Sensitivity Tests Solid Phase Microextraction Volatile Organic Compounds/*analysis/isolation & pur;
Notes:		"MedlineHossain, S M Zakir Bojko, Barbara Pawliszyn, Janusz eng Netherlands 2013/04/23 Anal Chim Acta. 2013 May 7; 776:41-9. doi: 10.1016/j.aca.2013.03.018. Epub 2013 Mar 15"