| Title: | Charged Residues Flanking the Transmembrane Domain of Two Related Toxin-Antitoxin System Toxins Affect Host Response |
| Author(s): | Holmes A; Sadlon J; Weaver K; |
| Address: | "Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South Dakota, Vermillion, SD 57069, USA" |
| ISSN/ISBN: | 2072-6651 (Electronic) 2072-6651 (Linking) |
| Abstract: | "A majority of toxins produced by type I toxin-antitoxin (TA-1) systems are small membrane-localized proteins that were initially proposed to kill cells by forming non-specific pores in the cytoplasmic membrane. The examination of the effects of numerous TA-1 systems indicates that this is not the mechanism of action of many of these proteins. Enterococcus faecalis produces two toxins of the Fst/Ldr family, one encoded on pheromone-responsive conjugative plasmids (Fst(pAD1)) and the other on the chromosome, Fst(EF0409). Previous results demonstrated that overexpression of the toxins produced a differential transcriptomic response in E. faecalis cells. In this report, we identify the specific amino acid differences between the two toxins responsible for the differential response of a gene highly induced by Fst(pAD1) but not Fst(EF0409). In addition, we demonstrate that a transporter protein that is genetically linked to the chromosomal version of the TA-1 system functions to limit the toxicity of the protein" |
| Keywords: | "Amino Acid Sequence Bacterial Toxins/genetics Enterococcus faecalis/*genetics Gene Expression Regulation, Bacterial Protein Domains/*genetics Toxin-Antitoxin Systems/*genetics Enterococcus faecalis Fst/Ldr family toxin 4 small protein toxins 3 type I toxi;" |
| Notes: | "MedlineHolmes, Andrew Sadlon, Jessie Weaver, Keith eng R21 AI140037/AI/NIAID NIH HHS/ AI140037/NH/NIH HHS/ Research Support, Non-U.S. Gov't Switzerland 2021/06/03 Toxins (Basel). 2021 May 1; 13(5):329. doi: 10.3390/toxins13050329" |
