| Title: | Analysis of defensive secretion of a milkweed bug Lygaeus equestris by 1D GC-MS and GCxGC-MS: sex differences and host-plant effect |
| Author(s): | Havlikova M; Bosakova T; Petschenka G; Cabala R; Exnerova A; Bosakova Z; |
| Address: | "Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic. Department of Insect Biotechnology, Justus-Liebig-University Giessen, Giessen, Germany. Toxicology Department, Institute of Forensic Medicine and Toxicology, General University Hospital in Prague, Prague, Czech Republic. Department of Zoology, Faculty of Science, Charles University, Prague, Czech Republic. alice.exnerova@natur.cuni.cz. Department of Analytical Chemistry, Faculty of Science, Charles University, Prague, Czech Republic. bosakova@natur.cuni.cz" |
| DOI: | 10.1038/s41598-020-60056-9 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "The composition of defensive secretion produced by metathoracic scent glands was analysed in males and females of the milkweed bug Lygaeus equestris (Heteroptera) using gas chromatography with mass spectrometric detection (GC-MS). The bugs were raised either on cardenolide-containing Adonis vernalis or on control sunflower seeds in order to determine whether the possibility to sequester cardenolides from their host plants would affect the composition of defensive scent-gland secretion. Profiles of the composition of defensive secretions of males and females raised on sunflower were closely similar, with predominant presence of (E)-2-octenal, (E)-2-octen-1-ol, decanal and 3-octen-1-ol acetate. The secretion of bugs raised on A. vernalis was more sexually dimorphic, and some chemicals e.g. (E,E)-2,4-hexadienyl acetate and 2-phenylethyl acetate were dominant in males, but absent in females. Compared to bugs from sunflower, the scent-gland secretion of bugs raised on A. vernalis was characterized by lower overall intensity of the peaks obtained for detected chemicals and by absence of some chemicals that have supposedly antipredatory function ((E)-2-hexenal, (E)-4-oxo-hex-2-enal, 2,4-octadienal). The results suggest that there might be a trade-off between the sequestration of defensive chemicals from host plants and their synthesis in metathoracic scent-glands" |
| Keywords: | "Acetates/analysis Adonis/*chemistry Aldehydes/analysis Animals Chromatography, Gas Female Gas Chromatography-Mass Spectrometry Helianthus/chemistry Heteroptera/*chemistry Male Phenylethyl Alcohol/analogs & derivatives/analysis Pheromones/analysis *Plant D;" |
| Notes: | "MedlineHavlikova, Martina Bosakova, Tereza Petschenka, Georg Cabala, Radomir Exnerova, Alice Bosakova, Zuzana eng Research Support, Non-U.S. Gov't England 2020/02/23 Sci Rep. 2020 Feb 20; 10(1):3092. doi: 10.1038/s41598-020-60056-9" |
