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Anal Chem

Title:		Fluorometric Biosniffer Camera 'Sniff-Cam' for Direct Imaging of Gaseous Ethanol in Breath and Transdermal Vapor
Author(s):		Arakawa T; Sato T; Iitani K; Toma K; Mitsubayashi K;
Address:		"Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University , 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University , 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8549, Japan"
Journal Title:		Anal Chem
Year:		2017
Volume:		20170331
Issue:		8
Page Number:		4495 - 4501
DOI:		10.1021/acs.analchem.6b04676
ISSN/ISBN:		1520-6882 (Electronic) 0003-2700 (Linking)
Abstract:		"Various volatile organic compounds can be found in human transpiration, breath and body odor. In this paper, a novel two-dimensional fluorometric imaging system, known as a 'sniffer-cam' for ethanol vapor released from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multiple ultraviolet light emitting diode (UV-LED) excitation sheet, an ADH enzyme immobilized mesh substrate and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed rapid and accurate responses and a visible measurement, which could lead to an analysis of metabolism function at real time in the near future"
Keywords:		"Alcohol Dehydrogenase/chemistry/metabolism Breath Tests Enzymes, Immobilized/chemistry/metabolism Ethanol/*analysis/metabolism *Fluorometry Gases/*chemistry Humans NAD/chemistry/metabolism Skin/chemistry/metabolism Ultraviolet Rays;"
Notes:		"MedlineArakawa, Takahiro Sato, Toshiyuki Iitani, Kenta Toma, Koji Mitsubayashi, Kohji eng Research Support, Non-U.S. Gov't 2017/04/01 Anal Chem. 2017 Apr 18; 89(8):4495-4501. doi: 10.1021/acs.analchem.6b04676. Epub 2017 Mar 31"