| Title: | [Determination of volatile nitrosamines in urine through gas chromatography-mass spectrometry] |
| Author(s): | Guo RZ; Wan YJ; Wu CJ; Zhang Y; Huang QH; Li HX; Wang X; Cao ZH; Mao YH; Shen J; Xia W; Li YY; Xu SQ; |
| Address: | "Department of Hygienic Analysis and Detection, Center for Disease Control and Prevention of Changjiang River Administration and Navigational Affairs, Ministry of Transport, Wuhan 430019, China" |
| Journal Title: | Zhonghua Yu Fang Yi Xue Za Zhi |
| ISSN/ISBN: | 0253-9624 (Print) 0253-9624 (Linking) |
| Abstract: | "OBJECTIVE: To establish a detection method based on gas chromatography-mass spectrometry (GC-MS) for concentrations of volatile nitrosamine compounds in urine, and apply it to the test of real samples. METHODS: Target compounds dichloromethane in urine samples was extracted with dichloromethane through liquid-liquid extraction, then the dichloromethane extract was filtrated, evaporated with nitrogen at 40 degrees C to dryness, and the volume was set with 0.2 ml dichloromethane. Analysis of nine volatile nitroso-compounds were performed with GC-MS under selected ion monitoring mode, external reference method was used for quantification, and the detection limit, repeatability and sensitivity were evaluated. In addition, nine volatile nitroso-compounds of 92 urine samples in a town of Anhui province were measured. RESULTS: A good linear range of 2 - 200 ng/ml (with correlation coefficient 0.9985 - 0.9999) were obtained for the above mentioned nine kinds of analyte, and the lowest examination concentration was 0.05 - 0.50 ng/ml. The addition standard recoveries were 68%-102% with the RSD of 0.4% - 5.5% (n = 3). The detection limits were 0.001 - 0.013 ng/ml urine. The detection rate of N-nitrosodimethylamine (NDMA), N-nitrosomethylethylamine (NMEA), N-nitrosodiethylamine (NDEA), N-nitrosodi-n-propylamine (NDPA), N-nitrosopyrrolidine (NPYR), N-nitrosomorpholine (NMOR), N-nitrosopiperidine (NPIP), N-nitrosodi-n-butylamine (NDBA) and N-nitrosodiphenylamine (NDPhA) were 71% (65), 74% (68), 65% (60), 80% (73), 92% (85), 78% (72), 76% (70), 87% (80), 98% (90), respectively, with the results (0.27 +/- 0.12), (0.75 +/- 0.29), (0.06 +/- 0.02), (0.16 +/- 0.07), (23.66 +/- 5.18), (1.01 +/- 0.35), (0.38 +/- 0.11), (2.47 +/- 0.52) and (15.13 +/- 3.48) nmol/g creatinine. CONCLUSIONS: A gas chromatography-mass spectrometry detect method was developed for low level volatile nitrosamines in urine samples" |
| Keywords: | *Gas Chromatography-Mass Spectrometry Humans Nitrosamines/*urine Urinalysis/*methods Volatile Organic Compounds/urine; |
| Notes: | "MedlineGuo, Run-zheng Wan, Yan-jian Wu, Chun-jiang Zhang, Yan Huang, Qi-hui Li, Hai-xia Wang, Xu Cao, Zhong-hou Mao, Yu-hua Shen, Jie Xia, Wei Li, Yuan-yuan Xu, Shun-qing chi English Abstract Research Support, Non-U.S. Gov't China 2013/07/23 Zhonghua Yu Fang Yi Xue Za Zhi. 2013 Mar; 47(3):270-3" |
