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Microorganisms

Title:		Kosakonia cowanii Ch1 Isolated from Mexican Chili Powder Reveals Growth Inhibition of Phytopathogenic Fungi
Author(s):		Gonzalez Espinosa J; Hernandez Gomez YF; Javier Martinez Y; Flores Gallardo FJ; Pacheco Aguilar JR; Ramos Lopez MA; Arvizu Gomez JL; Saldana Gutierrez C; Rodriguez Morales JA; Garcia Gutierrez MC; Amaro Reyes A; Alvarez Hidalgo E; Nunez Ramirez J; Hernandez Flores JL; Campos Guillen J;
Address:		"Facultad de Ciencias Naturales, Universidad Autonoma de Queretaro, Av. De las Ciencias S/N, Queretaro 76220, Mexico. Facultad de Quimica, Universidad Autonoma de Queretaro, Cerro de las Campanas S/N, Queretaro 76010, Mexico. Secretaria de Investigacion y Posgrado, Centro Nayarita de Innovacion y Transferencia de Tecnologia (CENITT), Universidad Autonoma de Nayarit, Tepic 63173, Mexico. Facultad de Ingenieria, Universidad Autonoma de Queretaro, Cerro de las Campanas S/N, Queretaro 76010, Mexico. Centro de Investigacion y de Estudios Avanzados del IPN, Irapuato 36824, Mexico"
Journal Title:		Microorganisms
Year:		2023
Volume:		20230705
Issue:		7
Page Number:		-
DOI:		10.3390/microorganisms11071758
ISSN/ISBN:		2076-2607 (Print) 2076-2607 (Electronic) 2076-2607 (Linking)
Abstract:		"Kosakonia cowanii strain Ch1 was isolated from Mexican chili powder, and the genome was sequenced. The genome was 4,765,544 bp in length, with an average G + C content of 56.22%, and a plasmid (pCh1) of 128,063 bp with an average G + C content of 52.50%. A phylogenetic analysis revealed a close relation with pathogenic strains; nevertheless, some virulence-related genes were absent, and this genetic characteristic may explain the fact that K. cowanii Ch1 behaved as a non-pathogenic strain when infection assays were performed on the leaves and fruits of Capsicum annuum L. Surprisingly, we observed that this bacterial strain had the ability to spread throughout serrano pepper seeds. Furthermore, K. cowanii Ch1 was evaluated for the production of volatile organic compounds (VOCs) against fungal pathogens, and the results showed that Alternaria alternata and Sclerotium rolfsii were inhibited in a radial mycelial growth assay by a mean rate of 70% and 64%, while Fusarium oxysporum was inhibited by only approximately 10%. Based on the headspace solid-phase microextraction combined with the gas chromatography mass spectrometry (HS-SPME-GC-MS), 67 potential VOCs were identified during the fermentation of K. cowanii Ch1 in TSA medium. From these VOCs, nine main compounds were identified based on relative peak area: dodecanoic acid; 3-hydroxy ethanol; 1-butanol-3-methyl; acetaldehyde; butanoic acid, butyl ester; cyclodecane; 2-butanone, 3-hydroxy; disulfide, dimethyl and pyrazine-2,5-dimethyl. Our findings show the potential of K. cowanii Ch1 for the biocontrol of fungal pathogens through VOCs production and reveal additional abilities and metabolic features as beneficial bacterial specie"
Keywords:		Kosaconia cowanii Ch1 biocontrol chili powder fungal pathogens genome sequencing volatile organic compounds;
Notes:		"PubMed-not-MEDLINEGonzalez Espinosa, Jacqueline Hernandez Gomez, Yoali Fernanda Javier Martinez, Yomaiko Flores Gallardo, Francisco Javier Pacheco Aguilar, Juan Ramiro Ramos Lopez, Miguel Angel Arvizu Gomez, Jackeline Lizzeta Saldana Gutierrez, Carlos Rodriguez Morales, Jose Alberto Garcia Gutierrez, Maria Carlota Amaro Reyes, Aldo Alvarez Hidalgo, Erika Nunez Ramirez, Jorge Hernandez Flores, Jose Luis Campos Guillen, Juan eng FONDEC-UAQ-2022; FOPER-2022/Universidad Autonoma de Queretaro/ Switzerland 2023/07/29 Microorganisms. 2023 Jul 5; 11(7):1758. doi: 10.3390/microorganisms11071758"