| Title: | Activation of the cerebellum by olfactory stimulation in sexually naive male rats |
| Author(s): | Garcia LI; Garcia-Banuelos P; Aranda-Abreu GE; Herrera-Meza G; Coria-Avila GA; Manzo J; |
| Address: | "Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico. Electronic address: luisgarcia@uv.mx. Centro de Investigaciones Cerebrales, Universidad Veracruzana, Xalapa, Veracruz, Mexico" |
| DOI: | 10.1016/j.nrl.2014.02.002 |
| ISSN/ISBN: | 1578-1968 (Electronic) 0213-4853 (Linking) |
| Abstract: | "INTRODUCTION: The cerebellum has been linked to multiple functions, such as motor control, cognition, memory, and emotional processing. As for its involvement in the sensory systems, the role of the cerebellum in the sense of smell remains unclear. We suggest that sexually naive male rats will present increased neuronal activity in the cerebellar vermis after being stimulated with almond odour or oestrous odour from receptive females. METHODS: We compared activity in the cerebellar vermis using Fos immunoreactivity after olfactory stimulation. Stimulation took place during 60 min in a cube-shaped acrylic chamber with a double bottom. Stimuli were clean woodchip bedding, bedding with almond extract, and bedding taken from a cage of receptive females. Male rats were subsequently anaesthetised with intraperitoneal sodium pentobarbital. Cerebellar tissue was fixed with paraformaldehyde for later immunohistochemical analysis. RESULTS: The number of Fos immunoreactive cells in all lobes of the cerebellar vermis was similar between groups stimulated with almond extract and with oestrous odour, and higher than in the clean woodchip group. CONCLUSIONS: Stimulation of the main olfactory system (almond) and the accessory system (oestrous odour) increases Fos protein production in the granular layer of the cortex of the cerebellar vermis in naive male rats" |
| Keywords: | "Animals Cerebellum/*metabolism Female Immunohistochemistry Male Neurons/metabolism Olfactory Bulb/cytology/*metabolism Proto-Oncogene Proteins c-fos/metabolism Rats Rats, Wistar Sex Attractants/physiology Sexual Behavior, Animal/*physiology Smell/*physiol;" |
| Notes: | "MedlineGarcia, L I Garcia-Banuelos, P Aranda-Abreu, G E Herrera-Meza, G Coria-Avila, G A Manzo, J eng spa Spain 2014/04/08 Neurologia. 2015 Jun; 30(5):264-9. doi: 10.1016/j.nrl.2014.02.002. Epub 2014 Apr 4" |
