| Title: | Analytical aspects of cyanobacterial volatile organic compounds for investigation of their production behavior |
| Author(s): | Fujise D; Tsuji K; Fukushima N; Kawai K; Harada K; |
| Address: | "Graduate School of Environmental and Human Science and Faculty of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku, Nagoya 468-8503, Japan. fujise-d@city.kawasaki.jp" |
| DOI: | 10.1016/j.chroma.2010.07.008 |
| ISSN/ISBN: | 1873-3778 (Electronic) 0021-9673 (Linking) |
| Abstract: | "In order to fully understand the role of volatile organic compounds (VOCs) under natural conditions, an adaptable analytical method was developed as the first step. beta-Ionone, beta-cyclocitral, 2-methyl-1-butanol and 3-methyl-1-butanol were simultaneously analyzed in addition to geosmin and 2-MIB using GC/MS with SPME. The slight modification of a known method allowed the simultaneous detection and quantification of these VOCs. The SIM of the 3-methyl-1-butanol was always accompanied by a shoulder peak, suggesting the presence of two compounds. In order to separate both compounds, the GC/MS conditions were optimized, and the additional peak was identified as 2-methyl-1-butanol by direct comparison of the authentic compound, indicating that the Microcystis strain always produces a mixture of 2-methyl-1-butanol and 3-methyl-1-butanol. Furthermore, it was found that 2-methyl-1-butanol and 3-methyl-1-butanol were predominant in the dissolved fractions. beta-Cyclocitral was easily oxidized to provide the oxidation product, 2,6,6-trimethylcyclohexene-1-carboxylic acid, which causes the blue color formation of cyanobacteria as a consequence of acid stress. The intact acid could be satisfactorily analyzed using the usual GC/MS without derivatization" |
| Keywords: | Butanols/chemistry Cyanobacteria/*chemistry Fresh Water/chemistry Gas Chromatography-Mass Spectrometry/*methods Solid Phase Microextraction/*methods Volatile Organic Compounds/*analysis/chemistry/isolation & purification; |
| Notes: | "MedlineFujise, Daiki Tsuji, Kiyomi Fukushima, Naoko Kawai, Kohei Harada, Ken-ichi eng Netherlands 2010/08/28 J Chromatogr A. 2010 Sep 24; 1217(39):6122-5. doi: 10.1016/j.chroma.2010.07.008. Epub 2010 Aug 24" |
