| Title: | "Investigating aroma diversity combining purge-and-trap, comprehensive two-dimensional gas chromatography, and mass spectrometry" |
| Author(s): | Franchina FA; Zanella D; Lazzari E; Stefanuto PH; Focant JF; |
| Address: | "Molecular System, Organic & Biological Analytical Chemistry Group, University of Liege, Liege, Belgium. Institute of Chemistry, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil" |
| ISSN/ISBN: | 1615-9314 (Electronic) 1615-9306 (Linking) |
| Abstract: | "Headspace gas chromatography is frequently used for aroma profiling thanks to its ability to naturally exploit the volatility of aroma compounds, and also to provide chemical information on sample composition. Its main advantages rely on simplicity, no use of solvent, amenability to automation, and the cleanliness of the extract. In the present contribution, the most effective sampling (dynamic extraction), separation (multidimensional gas chromatography), and detection (mass spectrometry) techniques for untargeted analysis are exploited in combination, showing their potential in unraveling aroma profiles in fruit beers. To complete the overall analytical process, a neat workflow for data analysis is discussed and used for the successful characterization and identification of five different beer flavors (berries, cherry, banana, apple, and peach). From the technical viewpoint, the coupling of purge-and-trap, comprehensive two-dimensional gas chromatography, and mass spectrometry makes the global methodology unique, and it is for the first time discussed. A (low-)flow modulation approach allowed for the full transfer into the second dimension with mass-spectrometry compatible flow (< 7 mL/min), avoiding the need of splitting before detection and making the overall method sensitive (1.2-5.2-fold higher signal to noise ratio compared to unmodulated gas chromatography conditions) and selective" |
| Keywords: | "Beer/*analysis Chromatography, Gas Mass Spectrometry Odorants/*analysis dynamic headspace sampling flow modulation GC x GC food authenticity time-of-flight mass spectrometry untargeted analysis;" |
| Notes: | "MedlineFranchina, Flavio Antonio Zanella, Delphine Lazzari, Eliane Stefanuto, Pierre-Hugues Focant, Jean-Francois eng 30897864/FWO/FNRS Belgium EOS/ Fund for Industry and Agricultural Research/ Germany 2019/11/02 J Sep Sci. 2020 May; 43(9-10):1790-1799. doi: 10.1002/jssc.201900902. Epub 2019 Nov 18" |
