« Previous Abstract"Identification of predominant aroma components of raw, dry roasted and oil roasted almonds"    Next AbstractAn invasive herbivorous fish (Siganus rivulatus) influences both benthic and planktonic microbes through defecation and nutrient excretion »

Naunyn Schmiedebergs Arch Pharmacol

Title:		"Differential inhibition and potentiation of chemoattractant-induced superoxide formation in human neutrophils by the cell-permeant analogue of cyclic GMP, N2,2'-O-dibutyryl guanosine 3':5'-cyclic monophosphate"
Author(s):		Ervens J; Schultz G; Seifert R;
Address:		"Institut fur Pharmakologie, Freie Universitat Berlin, Federal Republic of Germany"
Journal Title:		Naunyn Schmiedebergs Arch Pharmacol
Year:		1991
Volume:		343
Issue:		4
Page Number:		370 - 376
DOI:		10.1007/BF00179041
ISSN/ISBN:		0028-1298 (Print) 0028-1298 (Linking)
Abstract:		"Human neutrophils possess a superoxide (O2-)-forming NADPH oxidase which is activated by the chemoattractants, N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMet-Leu-Phe), complement C5a, platelet-activating factor and leukotriene B4. We studied the roles of cAMP and cGMP in the regulation of O2- formation using the cell-permeant analogues of cyclic nucleotides, N6,2'-O-dibutyryl adenosine 3':5'-cyclic monophosphate (Bt2cAMP) and N2,2'-O-dibutyryl guanosine 3':5'-cyclic monophosphate (Bt2cGMP). Bt2cAMP inhibited O2- formation induced by these chemoattractants to similar extents. Bt2cGMP as low as 10 mumol/l significantly inhibited O2- formation induced by fMet-Leu-Phe at a submaximally effective concentration (50 nmol/l), and Bt2cGMP was more effective in diminishing O2- formation than Bt2cAMP. In contrast, Bt2cGMP did not affect O2- formation induced by fMet-Leu-Phe at a maximally effective concentration (1 mumol/l). Bt2cGMP (0.1 and 1 mmol/l) enhanced O2- formation induced by 0.1 mumol/1 C5a by 23% and 49%, respectively, and Bt2cGMP antagonized inhibition of O2- formation caused by Bt2cAMP. Bt2cGMP inhibited platelet-activating factor-induced O2- formation to a lesser extent than Bt2cAMP and had no effect on that induced by leukotriene B4. Bt2cAMP and Bt2cGMP had no effect on O2- formation induced by NAF, gamma-hexachlorocyclohexane, phorbol myristate acetate, A 23187 and arachidonic acid. Our data suggest that: 1. Bt2cAMP generally inhibits chemoattractant-stimulated O2- formation. 2. Bt2cGMP inhibits fMet-Leu-Phe- and platelet-activating factor-stimulated O2- formation but potentiates C5a-induced O2- formation.(ABSTRACT TRUNCATED AT 250 WORDS)"
Keywords:		Bucladesine/*pharmacology Cyclic AMP/analogs & derivatives Cyclic GMP/analogs & derivatives Dibutyryl Cyclic GMP/*pharmacology Humans Neutrophils/*drug effects/metabolism Sex Attractants/*pharmacology Superoxides/*isolation & purification;
Notes:		"MedlineErvens, J Schultz, G Seifert, R eng Comparative Study Research Support, Non-U.S. Gov't Germany 1991/04/01 Naunyn Schmiedebergs Arch Pharmacol. 1991 Apr; 343(4):370-6. doi: 10.1007/BF00179041"