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FEBS Lett


Title:Expression of functional Raphanus sativus antifungal protein in yeast
Author(s):Alves AL; De Samblanx GW; Terras FR; Cammue BP; Broekaert WF;
Address:"F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium"
Journal Title:FEBS Lett
Year:1994
Volume:348
Issue:3
Page Number:228 - 232
DOI: 10.1016/0014-5793(94)00597-4
ISSN/ISBN:0014-5793 (Print) 0014-5793 (Linking)
Abstract:Rs-AFP2 is a 51 amino acid cysteine-rich peptide isolated from radish (Raphanus sativus) seeds that exhibits potent inhibitory activity against filamentous fungi. A cDNA clone encoding the Rs-AFP2 preprotein was modified by recombinant DNA methods to allow expression in the yeast Saccharomyces cerevisiae. This peptide was expressed in yeast as a fusion protein carrying at its N-terminus the prepro-sequences derived from the precursor of the yeast pheromone mating factor alpha 1. These sequences allow secretion of the biologically active peptide in a correctly processed form. Deletion of the mating factor alpha 1 pro-peptide drastically reduced the expression level of the peptide
Keywords:Amino Acid Sequence *Antifungal Agents Base Sequence Fusarium/drug effects *Gene Expression Gene Transfer Techniques Genetic Vectors Molecular Sequence Data Plant Proteins/chemistry/*genetics/pharmacology Plasmids Polymerase Chain Reaction Recombinant Fus;
Notes:"MedlineAlves, A L De Samblanx, G W Terras, F R Cammue, B P Broekaert, W F eng Research Support, Non-U.S. Gov't England 1994/07/18 FEBS Lett. 1994 Jul 18; 348(3):228-32. doi: 10.1016/0014-5793(94)00597-4"

 
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