| Title: | Epoxiconazole exposure affects terpenoid profiles of oilseed rape plantlets based on a targeted metabolomic approach |
| Author(s): | Durenne B; Blondel A; Druart P; Fauconnier ML; |
| Address: | "Bioengineering Unit, Life Science Department, Walloon Agricultural Research Centre, 5030, Gembloux, Belgium. b.durenne@cra.wallonie.be. Bioengineering Unit, Life Science Department, Walloon Agricultural Research Centre, 5030, Gembloux, Belgium. General and Organic Chemistry, Gembloux Agro-Bio Tech, University of Liege (ULiege), 5030, Gembloux, Belgium" |
| Journal Title: | Environ Sci Pollut Res Int |
| DOI: | 10.1007/s11356-019-05110-4 |
| ISSN/ISBN: | 1614-7499 (Electronic) 0944-1344 (Linking) |
| Abstract: | "Epoxiconazole is a broad-spectrum fungicide described as highly persistent in soil and as such can be considered as an abiotic agent like other problematic agrochemicals. Furthermore, the plant phenotyping tool involving non-invasive monitoring of plant-emitted volatile organic compounds (VOCs) may be useful in the identification of metabolic markers for abiotic stress. We therefore decided to profile the VOCs from secondary metabolism of oilseed rape through a dose-response experiment under several epoxiconazole concentrations (0, 0.01, 0.1 and 1 mg L(-1)). VOC collections of 35-day-old whole plantlets were performed through a dynamic headspace sampling technique under defined and controlled conditions. The plantlets grew freely within a home-made, laboratory and high-throughput glass chamber without any disturbance. Putative metabolic markers were analysed using a targeted metabolomic approach based on TD-GC-MS method coupled with data acquisition in SIM mode in order to focus on terpenes and sulphur-containing volatiles. Chromatograms of emitted terpenes were achieved accurately for the 35-day-old oilseed rape plantlets. We also analysed the presence of sulphur-containing volatiles in samples of shoot and root tissues using an innovative DHS-TD-GC-MS method, but no difference was found between qualitative profiles. Nevertheless, we demonstrated through this experiment that sesquiterpenes such as beta-elemene and (E,E)-alpha-farnesene are involved in epoxiconazole dose-response. In particular, (E,E)-alpha-farnesene could serve as a metabolic marker of fungicide exposure for oilseed rape plantlets" |
| Keywords: | "Brassica napus/drug effects/metabolism/*physiology Epoxy Compounds/*toxicity Gas Chromatography-Mass Spectrometry Metabolome/drug effects/physiology Metabolomics Plant Extracts/metabolism Sesquiterpenes Soil Pollutants/*toxicity Stress, Physiological Terp;" |
| Notes: | "MedlineDurenne, Bastien Blondel, Alodie Druart, Philippe Fauconnier, Marie-Laure eng Germany 2019/04/24 Environ Sci Pollut Res Int. 2019 Jun; 26(17):17362-17372. doi: 10.1007/s11356-019-05110-4. Epub 2019 Apr 23" |
