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PLoS One

Title:		Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant
Author(s):		Durand N; Carot-Sans G; Chertemps T; Bozzolan F; Party V; Renou M; Debernard S; Rosell G; Maibeche-Coisne M;
Address:		"UMR-A 1272 UPMC-INRA Physiologie de l'Insecte, Universite Pierre et Marie Curie and INRA, Paris and Versailles, France"
Journal Title:		PLoS One
Year:		2010
Volume:		20101129
Issue:		11
Page Number:		e15026 -
DOI:		10.1371/journal.pone.0015026
ISSN/ISBN:		1932-6203 (Electronic) 1932-6203 (Linking)
Abstract:		"BACKGROUND: Carboxyl/cholinesterases (CCEs) are highly diversified in insects. These enzymes have a broad range of proposed functions, in neuro/developmental processes, dietary detoxification, insecticide resistance or hormone/pheromone degradation. As few functional data are available on purified or recombinant CCEs, the physiological role of most of these enzymes is unknown. Concerning their role in olfaction, only two CCEs able to metabolize sex pheromones have been functionally characterized in insects. These enzymes are only expressed in the male antennae, and secreted into the lumen of the pheromone-sensitive sensilla. CCEs able to hydrolyze other odorants than sex pheromones, such as plant volatiles, have not been identified. METHODOLOGY: In Spodoptera littoralis, a major crop pest, a diversity of antennal CCEs has been previously identified. We have employed here a combination of molecular biology, biochemistry and electrophysiology approaches to functionally characterize an intracellular CCE, SlCXE10, whose predominant expression in the olfactory sensilla suggested a role in olfaction. A recombinant protein was produced using the baculovirus system and we tested its catabolic properties towards a plant volatile and the sex pheromone components. CONCLUSION: We showed that SlCXE10 could efficiently hydrolyze a green leaf volatile and to a lesser extent the sex pheromone components. The transcript level in male antennae was also strongly induced by exposure to this plant odorant. In antennae, SlCXE10 expression was associated with sensilla responding to the sex pheromones and to plant odours. These results suggest that a CCE-based intracellular metabolism of odorants could occur in insect antennae, in addition to the extracellular metabolism occurring within the sensillar lumen. This is the first functional characterization of an Odorant-Degrading Enzyme active towards a host plant volatile"
Keywords:		"Animals Blotting, Western Carboxylesterase/genetics/*metabolism Cell Line Female Gene Expression Regulation, Developmental Host-Parasite Interactions Hydrolysis In Situ Hybridization Insect Proteins/genetics/*metabolism Kinetics Male Odorants Olfactory Re;"
Notes:		"MedlineDurand, Nicolas Carot-Sans, Gerard Chertemps, Thomas Bozzolan, Francoise Party, Virginie Renou, Michel Debernard, Stephane Rosell, Gloria Maibeche-Coisne, Martine eng Research Support, Non-U.S. Gov't 2010/12/03 PLoS One. 2010 Nov 29; 5(11):e15026. doi: 10.1371/journal.pone.0015026"