| Title: | Moth oviposition shapes the species-specific transcriptional and phytohormonal response of Nicotiana attenuata to larval feeding |
| Author(s): | Drok S; Bandoly M; Stelzer S; Lortzing T; Steppuhn A; |
| Address: | "Freie Universitat of Berlin/Institute of Biology/Dahlem Centre of Plant Sciences, Laboratory of Molecular Ecology, Albrecht-Thaer Weg 6, Berlin, 14195, Germany. Freie Universitat of Berlin/Institute of Biology/Dahlem Centre of Plant Sciences, Laboratory of Molecular Ecology, Albrecht-Thaer Weg 6, Berlin, 14195, Germany. a.steppuhn@fu-berlin.de" |
| DOI: | 10.1038/s41598-018-28233-z |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Oviposition by lepidopteran herbivores on Nicotiana attenuata primes plant defence responses that are induced by the feeding larvae. While oviposition by both the generalist Spodoptera exigua and the specialist Manduca sexta primes the production of defensive phenylpropanoids, their larvae are differentially affected. We investigate here the impact of prior oviposition on the transcriptome and phytohormone levels of plants that were later attacked by larvae to find regulatory signals of this priming. In a full-factorial design, we evaluated the effects of oviposition and herbivory by both species. Oviposition alone had only subtle effects at the transcriptional level. Laval feeding alone induced species-specific plant responses. Larvae of the generalist regulated phytohormones and gene expression stronger than larvae of the specialist. A day after larvae started to feed, we detected no significant alterations of the plant's response to larval feeding due to prior oviposition by conspecific moths. Yet, oviposition by each of the species profoundly influenced the plant's transcriptional and phytohormonal response to feeding larvae of the other species. Remarkably, the species-specific plant responses to larval feeding shifted towards the response normally elicited by larvae of the ovipositing species. Thus, plants may already recognise an insect's identity upon its oviposition" |
| Keywords: | "Animals Feeding Behavior/*physiology Female Gene Expression Profiling Gene Expression Regulation, Plant Herbivory Larva/drug effects/*physiology Manduca/physiology Moths/*physiology Oviposition/drug effects/*physiology Plant Growth Regulators/*pharmacolog;" |
| Notes: | "MedlineDrok, Sylvia Bandoly, Michele Stelzer, Sandra Lortzing, Tobias Steppuhn, Anke eng SFB973 - Project B2/Deutsche Forschungsgemeinschaft (German Research Foundation)/International stipend for M. Bandoly/Deutsche Bundesstiftung Umwelt (German Federal Environmental Foundation)/International Research Support, Non-U.S. Gov't England 2018/07/08 Sci Rep. 2018 Jul 6; 8(1):10249. doi: 10.1038/s41598-018-28233-z" |
