« Previous Abstract"Development of an efficient trapping system for New Zealand flower thrips, Thrips obscuratus"    Next Abstract[A method for detection of volatile organic halogenated compounds in human and animal tissues (author's transl)] »

J Breath Res

Title:		Measurement of exhaled volatile organic compounds from patients with chronic obstructive pulmonary disease (COPD) using closed gas loop GC-IMS and GC-APCI-MS
Author(s):		Allers M; Langejuergen J; Gaida A; Holz O; Schuchardt S; Hohlfeld JM; Zimmermann S;
Address:		"Institute of Electrical Engineering and Measurement Technology, Leibniz Universitat Hannover, Hannover, Germany"
Journal Title:		J Breath Res
Year:		2016
Volume:		20160408
Issue:		2
Page Number:		26004 -
DOI:		10.1088/1752-7155/10/2/026004
ISSN/ISBN:		1752-7163 (Electronic) 1752-7155 (Linking)
Abstract:		"Due to its high sensitivity, compact size and low cost ion mobility spectrometry (IMS) has the potential to become a point-of-care breath analyzer. Therefore, we developed a prototype of a compact, closed gas loop IMS with gas chromatographic (GC) pre-separation and high resolving power of R = 90. In this study, we evaluated the performance of this GC-IMS under clinical conditions in a COPD study to find correlations between VOCs (10 ppbv to 1 ppmv) and COPD. Furthermore, in order to investigate possible correlations between ultra-low concentrated breath VOCs (0.1 pptv to 1 ppbv) and COPD, a modified mass spectrometer (MS) with atmospheric pressure chemical ionization (APCI) and GC pre-separation (GC-APCI-MS) was used. The GC-IMS has been used in 58 subjects (21 smokers with moderate COPD, 12 ex-smokers with COPD, 16 healthy smokers and 9 non-smokers). GC-APCI-MS data were available for 94 subjects (21 smokers with moderate COPD, 25 ex-smokers with COPD, 25 healthy smokers and 23 non-smokers). For 44 subjects, a comparison between GC-IMS and GC-APCI-MS data could be performed. Due to service intervals, subject availability and corrupt data, patient numbers were different for GC-APCI-MS and GC-IMS measurements. Using GC-IMS, three VOCs have been found showing a significant difference between healthy controls and patients with COPD. In the GC-APCI-MS data, we only observed one distinctive VOC, which has been identified as 2-pentanone. This proof-of-principle study shows the potential of our high-resolution GC-IMS in the clinical environment. Due to different linear dynamic response ranges, the data of GC-IMS and GC-APCI-MS were only comparable to a limited extent"
Keywords:		"Adult Aged Breath Tests/*methods Chromatography, Gas/*methods Exhalation Female Humans Male Mass Spectrometry/*methods Middle Aged Point-of-Care Systems Pulmonary Disease, Chronic Obstructive/*physiopathology Volatile Organic Compounds/*analysis Young Adu;"
Notes:		"MedlineAllers, M Langejuergen, J Gaida, A Holz, O Schuchardt, S Hohlfeld, J M Zimmermann, S eng England 2016/04/09 J Breath Res. 2016 Apr 8; 10(2):026004. doi: 10.1088/1752-7155/10/2/026004"