| Title: | "Effects of academic stress on the levels of oral volatile sulfur compounds, halitosis-related bacteria and stress biomarkers of healthy female undergraduate students" |
| Author(s): | De Lima PO; Nani BD; Rolim GS; Groppo FC; Franz-Montan M; Alves De Moraes AB; Cogo-Muller K; Marcondes FK; |
| Address: | "Department of Biosciences, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil. These authors contributed equally to this work" |
| ISSN/ISBN: | 1752-7163 (Electronic) 1752-7155 (Linking) |
| Abstract: | "To investigate whether academic stress changes the salivary microbiota and its relationship with salivary parameters, evaluating the effects on the production of volatile sulfur compounds (VSCs) in healthy women. Fifty-five women who were enrolled in a four-year Dentistry course were assessed for academic stress by the questionnaire Maslach Burnout Inventory-Student Survey and were then classified into 'Not Stressed' and 'Stressed' groups. Cortisol and alpha-amylase (AA) were measured as physiological stress biomarkers. Oral Chroma gas chromatograph was used to measure concentrations of hydrogen sulfide (H(2)S), methyl mercaptan and dimethyl sulfide. Salivary proteins were evaluated by western blot. Quantitative polymerase chain reaction was used to identify the salivary amounts of halitosis-associated bacteria. The 'Stressed' volunteers showed higher levels of H(2)S, AA, Fusobacterium nucleatum and total bacteria, compared to the 'Not Stressed' individuals (p < 0.05; Mann-Whitney test). Salivary proteins showed no differences between groups (p > 0.05; Mann-Whitney test). Academic stress was positively correlated with H(2)S, total bacteria and F. nucleatum counts, while F. nucleatum was positively correlated with AA. H(2)S showed positive correlations with AA and Solobacterium moorei (p < 0.05; Spearman correlation). Beta-defensin (BD) presented negative correlations with H(2)S and S. moorei (p < 0.05; Spearman correlation). Academic stress increased salivary F. nucleatum and total bacteria, as well as AA activity. The protein BD showed important correlations with bacteria and VSC. These changes appeared to be accountable for increased H(2)S production in the stressed women" |
| Keywords: | "Adolescent Bacteria/metabolism Biomarkers/*analysis Breath Tests Female Halitosis/*microbiology Humans Hydrogen Sulfide/analysis Mouth/*microbiology Salivary Proteins and Peptides/metabolism Stress, Psychological/*microbiology *Students Sulfur Compounds/*;" |
| Notes: | "MedlineDe Lima, Patricia Oliveira Nani, Bruno Dias Rolim, Gustavo Satollo Groppo, Francisco Carlos Franz-Montan, Michelle Alves De Moraes, Antonio Bento Cogo-Muller, Karina Marcondes, Fernanda Klein eng Research Support, Non-U.S. Gov't England 2020/05/20 J Breath Res. 2020 Jul 3; 14(3):036005. doi: 10.1088/1752-7163/ab944d" |
