| Title: | Effect of prior drought and pathogen stress on Arabidopsis transcriptome changes to caterpillar herbivory |
| Author(s): | Davila Olivas NH; Coolen S; Huang P; Severing E; van Verk MC; Hickman R; Wittenberg AH; de Vos M; Prins M; van Loon JJ; Aarts MG; Van Wees SC; Pieterse CM; Dicke M; |
| Address: | "Laboratory of Entomology, Wageningen University, PO Box 16, 6700 AA, Wageningen, the Netherlands. Plant-Microbe Interactions, Department of Biology, Utrecht University, PO Box 800.56, 3508 TB, Utrecht, the Netherlands. Laboratory of Genetics, Wageningen University, PO Box 16, 6700 AA, Wageningen, the Netherlands. Bioinformatics, Utrecht University, PO Box 800.56, 3508 TB, Utrecht, the Netherlands. Keygene N.V., PO Box 216, 6700 AE, Wageningen, the Netherlands" |
| ISSN/ISBN: | 1469-8137 (Electronic) 0028-646X (Linking) |
| Abstract: | "In nature, plants are exposed to biotic and abiotic stresses that often occur simultaneously. Therefore, plant responses to combinations of stresses are most representative of how plants respond to stresses. We used RNAseq to assess temporal changes in the transcriptome of Arabidopsis thaliana to herbivory by Pieris rapae caterpillars, either alone or in combination with prior exposure to drought or infection with the necrotrophic fungus Botrytis cinerea. Pre-exposure to drought stress or Botrytis infection resulted in a significantly different timing of the caterpillar-induced transcriptional changes. Additionally, the combination of drought and P. rapae induced an extensive downregulation of A. thaliana genes involved in defence against pathogens. Despite a more substantial growth reduction observed for plants exposed to drought plus P. rapae feeding compared with P. rapae feeding alone, this did not affect weight increase of this specialist caterpillar. Plants respond to combined stresses with phenotypic and transcriptional changes that differ from the single stress situation. The effect of a previous exposure to drought or B. cinerea infection on transcriptional changes to caterpillars is largely overridden by the stress imposed by caterpillars, indicating that plants shift their response to the most recent stress applied" |
| Keywords: | "Animals Arabidopsis/*genetics/physiology Botrytis/physiology Butterflies/physiology Droughts *Gene Expression Regulation, Plant Herbivory Plant Diseases/*microbiology Stress, Physiological *Transcriptome Botrytis cinerea Pieris rapae RNAseq abiotic stress;" |
| Notes: | "MedlineDavila Olivas, Nelson H Coolen, Silvia Huang, Pingping Severing, Edouard van Verk, Marcel C Hickman, Richard Wittenberg, Alexander H J de Vos, Martin Prins, Marcel van Loon, Joop J A Aarts, Mark G M van Wees, Saskia C M Pieterse, Corne M J Dicke, Marcel eng Research Support, Non-U.S. Gov't England 2016/02/06 New Phytol. 2016 Jun; 210(4):1344-56. doi: 10.1111/nph.13847. Epub 2016 Feb 5" |
