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Nat Prod Res

Title:		Effect of extraction technique on the content and antioxidant activity of crude extract of Anacyclus clavatus flowers and their essential oil composition
Author(s):		Aliboudhar H; Tigrine-Kordjani N;
Address:		"a Laboratory of Functional Organic Analysis, Faculty of Chemistry, University of Sciences and Technology Houari Boumediene , BP32, El-Alia, Bab-Ezzouar, Algiers 16111 , Algeria"
Journal Title:		Nat Prod Res
Year:		2014
Volume:		20140812
Issue:		23
Page Number:		2140 - 2149
DOI:		10.1080/14786419.2014.927872
ISSN/ISBN:		1478-6427 (Electronic) 1478-6419 (Linking)
Abstract:		"Anacyclus clavatus is a plant used as food and remedy. The objective of this work was to study the effect of extraction technique on the antioxidant property, total phenol and flavonoid contents of crude extracts from A. clavatus flowers and their essential oil composition. 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, ferric-reducing power, beta-carotene and total antioxidant capacity assays have demonstrated the significant antioxidant ability of different crude extracts obtained by using the following extraction methods: Soxhlet, microwave heating, heat reflux (HRE) and maceration. The activity of the extract obtained by HRE was the highest (112.06 +/- 2.89 mug/mL) evaluated by the DPPH assay. Extraction of essential oil was performed by microwave-assisted hydro-distillation (MAHD) and by hydro-distillation (HD). A significant difference was observed in both essential oils, despite the common main family and major constituents, such as artemisia ketone (10.0 +/- 0.8% for MAHD vs. 6.5 +/- 0.5 for HD) and pinocarvone (4.1 +/- 0.4% for MAHD vs. 1.1 +/- 0.1% for HD)"
Keywords:		"Algeria Algorithms Antioxidants/chemistry/isolation & purification/*pharmacology Asteraceae/*chemistry Biphenyl Compounds/pharmacology Distillation Flavonoids/chemistry Flowers/*chemistry Gas Chromatography-Mass Spectrometry Microwaves Oils, Volatile/*che;"
Notes:		"MedlineAliboudhar, Hamza Tigrine-Kordjani, Nacera eng England 2014/08/15 Nat Prod Res. 2014; 28(23):2140-9. doi: 10.1080/14786419.2014.927872. Epub 2014 Aug 12"